Miller R D, Calkins C E
Department of Microbiology, Thomas Jefferson University Philadelphia, Pennsylvania 19107.
Immunology. 1988 Apr;63(4):625-30.
The in vitro anti-self erythrocyte antibody response of NZB spleen cells appears to be influenced directly by T cells. Thy-1+, L3T4+ helper T cells are required for: (i) the generation in vitro of MRBC-specific IgM and IgG AFC by spleen cells from 'autoimmune' (9-12-month old) NZB mice and (ii) the generation in vitro of MRBC-specific IgM and IgG AFC by spleen cells depleted of suppressor cells from pre-autoimmune (2-3-months-old) NZB mice which show no clinical signs of an anti-MRBC response. It is evident from the present and previous studies that the anti-MRBC autoantibody response is regulated in pre-autoimmune spleen cell populations by Ly2+ T cells. Ly2-T cells from both pre-autoimmune and autoimmune mice in sufficient numbers can overcome this normal regulation and promote the anti-MRBC response in cultures of unfractionated pre-autoimmune spleen cells. Ly2- T cells isolated from autoimmune NZB mice were consistently more active in this than the Ly2- T cells isolated from pre-autoimmune mice, suggesting an enrichment of MRBC-reactive Ly2- T cells in autoimmune NZB mice. The Ly2- T cells from autoimmune NZB mice greatly enhance the autoimmune anti-MRBC response relative to a modest enhancement of the response to a foreign antigen, SRBC, produced by the same cells. These data indicate that T cells play an important role both in supporting the autoantibody response to MRBC and in disrupting tolerance, leading to autoimmunity in NZB mice.
NZB脾细胞的体外抗自身红细胞抗体反应似乎直接受T细胞影响。Thy-1⁺、L3T4⁺辅助性T细胞对于以下情况是必需的：(i) 由“自身免疫性”（9至12月龄）NZB小鼠的脾细胞在体外产生针对MRBC的特异性IgM和IgG抗体形成细胞（AFC）；(ii) 由来自自身免疫前期（2至3月龄）NZB小鼠且已去除抑制性细胞的脾细胞在体外产生针对MRBC的特异性IgM和IgG AFC，这些小鼠没有抗MRBC反应的临床迹象。从目前及先前的研究可以明显看出，抗MRBC自身抗体反应在自身免疫前期脾细胞群体中受Ly2⁺ T细胞调节。来自自身免疫前期和自身免疫性小鼠的足够数量的Ly2⁻ T细胞能够克服这种正常调节，并促进未分级的自身免疫前期脾细胞培养物中的抗MRBC反应。从自身免疫性NZB小鼠分离的Ly2⁻ T细胞在这方面始终比从自身免疫前期小鼠分离的Ly2⁻ T细胞更活跃，这表明自身免疫性NZB小鼠中富含对MRBC有反应的Ly2⁻ T细胞。相对于相同细胞对异源抗原SRBC产生的反应的适度增强，来自自身免疫性NZB小鼠的Ly2⁻ T细胞极大地增强了自身免疫性抗MRBC反应。这些数据表明，T细胞在支持对MRBC的自身抗体反应以及破坏耐受性从而导致NZB小鼠自身免疫方面都起着重要作用。
