Assis Patricia, Carvalho Carlos Eduardo, Silva Marcelo Soares, Ribeiro Bruna, Carvalho Maria da Gloria
Laboratory of Molecular Pathology, Pathological Anatomy Service and Pathology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil.
Serviço de Anatomia Patológica, Subsolo - sala 09 (Citopatologia), Hospital Universitário Clementino Fraga Filho, UFRJ, Rio de Janeiro, RJ, Brazil.
Transpl Infect Dis. 2018 Aug;20(4):e12901. doi: 10.1111/tid.12901. Epub 2018 May 7.
Although identifying cytological viral inclusions (decoy cells) in the urine is relatively easy, distinguishing between Polyomaviruses BKV and JCV is not possible. Few studies have been published regarding JCV detection in kidney transplant recipients.
To evaluate the incidence of BKV and JCV DNA in archival slides of urine cytospin material from renal transplant patients.
A total of 44 urine specimens were evaluated cytologically for the presence of viral inclusions (decoy cells) and by nested polymerase chain reaction to differentiate between JCV and BKV in DNA isolated from archival slides of urine cytospin material.
Of the 44 urine specimen donors, 9 (20.5%) patients had at least 1 sample with alterations suggestive of or compatible with viral infection (decoy cells), and 3 had urine samples with cellular atypias/neoplasias. Additionally, 24/44 (54.5%) patients had PCR-positive DNA for Polyomavirus in at least 1 sample, including 11/44 who were positive for BKV (25%) and 16/44 who were positive for JCV (36.36%), with 3 (6.8%) patients showing viral coinfection. Regarding transplantation time, only JCV was statistically significant (P = .019) for periods longer than 10 years.
The results highlight the potential use of archival slides of urine cytospin material to differentiate BKV and JCV and demonstrate the importance of improved JCV detection for later kidney transplant recipients.
虽然在尿液中识别细胞学病毒包涵体（诱饵细胞）相对容易，但区分多瘤病毒BKV和JCV却不可能。关于肾移植受者中JCV检测的研究发表较少。
评估肾移植患者尿液细胞涂片存档玻片材料中BKV和JCV DNA的发生率。
对44份尿液标本进行细胞学评估，以检测病毒包涵体（诱饵细胞）的存在，并通过巢式聚合酶链反应区分从尿液细胞涂片存档玻片材料中分离的DNA中的JCV和BKV。
在44名尿液标本供者中，9名（20.5%）患者至少有1份样本出现提示或符合病毒感染的改变（诱饵细胞），3名患者尿液样本出现细胞异型性/肿瘤形成。此外，24/44（54.5%）患者至少有1份样本的多瘤病毒PCR检测DNA呈阳性，其中11/44（25%）为BKV阳性，16/44（36.36%）为JCV阳性，3名（6.8%）患者出现病毒合并感染。关于移植时间，仅JCV在超过10年的时间段内具有统计学意义（P = 0.019）。
结果突出了尿液细胞涂片存档玻片材料在区分BKV和JCV方面的潜在用途，并证明了改善JCV检测对晚期肾移植受者的重要性。
