[Study on the immune response of mice receiving bone allografts].

The immune response of bone allografts has not been well understood. The present study was performed to examine the MLC response, CML activity, and alloantibody production of inbred mice receiving fresh and frozen bone allografts. A) Fresh allografts: High MLC response was observed in an early phase of the culture, suggesting the activation and proliferation of helper T cells. Percent cytotoxicity of CML assay was 24% on the 9th day after grafting, indicating the production of cytotoxic T cells. High alloantibody titer was observed on the 30th day after grafting, further indicating the production of the cytotoxic alloantibody. B) Frozen allografts: No significant difference was observed between frozen allografts and fresh isografts (control group) in these immunological responses. These results indicated that the antigenicity of the frozen bone decreased greatly so that matching of the major histocompatibility complex may not be required.