Sogawa Kazuyuki, Takahashi Yuria, Shibata Yui, Satoh Mamoru, Kodera Yoshio, Nomura Fumio, Tanaka Toshio, Sato Hironori, Yamaide Fumiya, Nakano Taiji, Iwahashi Kazuhiko, Sugita-Konishi Yoshiko, Shimada Akinori, Shimojo Naoki
Department of Biochemistry, School of Life and Environmental Science, Azabu University, Sagamihara, Japan.
Divisions of Clinical Mass Spectrometry and Clinical Genetics, Chiba University Hospital, Chiba, Japan.
Int Arch Allergy Immunol. 2018;176(3-4):189-197. doi: 10.1159/000488144. Epub 2018 Apr 18.
Food allergy is a serious health issue affecting roughly 4% of children, with a substantial effect on quality of life. Chicken egg allergy is frequently observed in infants. Therefore, some of them have to exclude hen's eggs from their daily diet to avoid allergenic symptoms. Hen's egg is composed of 2 soluble parts; one is egg white, which has been characterized as the major source of allergenicity, while the other is egg yolk, which is estimated as a miner source. Only 2 allergens from egg yolk, α-livetin (Gal d 5) and YGP42 (Gal d 6), have been described to date.
Sera from 53 patients allergic to hen's eggs and 2 patients allergic to sesame were obtained from the Department of Pediatrics, Chiba University Hospital. The study was performed using SDS-PAGE, IgE immunoblotting, and dot blotting.
Seven bands of egg yolk were detected by IgE immunoblotting. Out of these bands, a possible new allergen was further characterized by LC-MS/MS. The 33-kDa band was identified as yolk glycoprotein (YGP40) by LC-MS/MS. A total of 21 of the 53 patients (47%) had YGP40 detected by dot blotting.
We identified YGP40 as a new hen's egg yolk allergen and detected 4 sites of YGP40 as linear epitopes.
食物过敏是一个严重的健康问题，大约影响4%的儿童，对生活质量有重大影响。鸡蛋过敏在婴儿中很常见。因此，其中一些婴儿不得不从日常饮食中排除鸡蛋以避免过敏症状。鸡蛋由两个可溶部分组成；一个是蛋清，它被认为是主要的过敏原来源，另一个是蛋黄，据估计是次要来源。迄今为止，仅描述了来自蛋黄的两种过敏原，即α-卵黄磷蛋白（Gal d 5）和YGP42（Gal d 6）。
从千叶大学医院儿科获得53例对鸡蛋过敏患者和2例对芝麻过敏患者的血清。使用SDS-PAGE、IgE免疫印迹和斑点印迹进行研究。
通过IgE免疫印迹检测到蛋黄的七条条带。在这些条带中，通过液相色谱-串联质谱（LC-MS/MS）进一步鉴定了一种可能的新过敏原。通过LC-MS/MS将33 kDa条带鉴定为卵黄糖蛋白（YGP40）。53例患者中有21例（47%）通过斑点印迹检测到YGP40。
我们将YGP40鉴定为一种新的鸡蛋黄过敏原，并检测到YGP40的4个线性表位位点。
