大鼠肾皮质细胞膜中细胞体积敏感性钠-ATP酶活性
Cell volume-sensitive Na+-ATPase activity in rat kidney cortex cell membranes.
作者信息
Proverbio F, Duque J A, Proverbio T, Marín R
机构信息
Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
出版信息
Biochim Biophys Acta. 1988 Jun 7;941(1):107-10. doi: 10.1016/0005-2736(88)90219-2.
A ouabain-insensitive, K+-independent, sodium pump, has been demonstrated in guinea-pig and rat kidney proximal tubular cells. This pump is thought to be distinct from the ouabain-sensitive Na+/K+ pump. We present evidence here indicating the modulation of the biochemical expression of the Na+ pump, i.e. the ouabain-insensitive Na+-ATPase, by the cell volume in rat kidney proximal tubular cells. Thus, basolateral plasma membranes from swollen cells show a ouabain-insensitive Na+-ATPase activity 10-times higher than that in membranes from control cells. If the swollen cells recover their volume, the activity decreases ten times to control values. The ouabain-sensitive Na+/K+-ATPase is not affected by changes in the cell volume.
在豚鼠和大鼠肾近端小管细胞中已证实存在一种对哇巴因不敏感、与钾离子无关的钠泵。这种泵被认为与对哇巴因敏感的钠钾泵不同。我们在此提供证据表明，大鼠肾近端小管细胞的细胞体积可调节钠泵的生化表达，即对哇巴因不敏感的钠 - 腺苷三磷酸酶（Na⁺ - ATPase）。因此，肿胀细胞的基底外侧质膜显示出的对哇巴因不敏感的钠 - 腺苷三磷酸酶活性比对照细胞质膜中的活性高10倍。如果肿胀细胞恢复其体积，该活性会降至对照值的十分之一。对哇巴因敏感的钠钾 - 腺苷三磷酸酶不受细胞体积变化的影响。
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