Ramsdell F J, Gray J D, Golub S H
Department of Microbiology, UCLA School of Medicine.
Cell Immunol. 1988 Jun;114(1):209-21. doi: 10.1016/0008-8749(88)90267-5.
Human thymocytes are devoid of NK cells but develop lymphokine-activated killer (LAK) activity after culture with recombinant interleukin-2 (rIL-2). The most active precursor for this activity appears to be a CD3-negative cell. The purpose of these studies was to compare the phenotype and functional activities of thymocyte and peripheral blood lymphocyte (PBL) LAK cells. Following culture, rIL-2-activated thymocytes resemble PBL-generated LAk and PBL NK cells. For each of these populations, lytic activity is highest in NKH-1-positive cells. Two-color fluorescence of each population also indicates that NKH-1+ cells are highly granular, as measured by staining with the lysosomotropic vital dye quinacrine. PBL, PBL-derived LAK cells, and thymus-derived LAK cells have a portion of cells that express both CD3 and NKH-1. However, approximately 60-80% of NKH-1+ cells lack detectable CD3. This suggests that both CD3+ and CD3- cells may be capable of LAK activity. Thymic-derived LAK cells respond to interferon in a manner very similar to NK and PBL-derived LAK cells, but lack the NK-associated CD16 antigen. Thus, despite the absence of NK cells in the thymus, it is possible to generate thymocyte LAK activity which bears a strong resemblance to LAK activity derived from peripheral blood lymphocytes.
人类胸腺细胞缺乏自然杀伤（NK）细胞，但在用重组白细胞介素-2（rIL-2）培养后会产生淋巴因子激活的杀伤（LAK）活性。这种活性最活跃的前体似乎是CD3阴性细胞。这些研究的目的是比较胸腺细胞和外周血淋巴细胞（PBL）LAK细胞的表型和功能活性。培养后，rIL-2激活的胸腺细胞类似于PBL产生的LAK细胞和PBL NK细胞。对于这些细胞群体中的每一个，NKH-1阳性细胞的裂解活性最高。每个细胞群体的双色荧光还表明，通过用溶酶体亲和活性染料喹吖因染色测量，NKH-1+细胞高度颗粒化。PBL、PBL来源的LAK细胞和胸腺来源的LAK细胞都有一部分细胞同时表达CD3和NKH-1。然而，大约60-80%的NKH-1+细胞缺乏可检测到的CD3。这表明CD3+和CD3-细胞都可能具有LAK活性。胸腺来源的LAK细胞对干扰素的反应方式与NK和PBL来源的LAK细胞非常相似，但缺乏与NK相关的CD16抗原。因此，尽管胸腺中不存在NK细胞，但有可能产生与外周血淋巴细胞来源的LAK活性非常相似的胸腺细胞LAK活性。
