麻醉剂与离子通道相互作用的溶液核磁共振研究。
Solution NMR Studies of Anesthetic Interactions with Ion Channels.
作者信息
Bondarenko Vasyl, Wells Marta, Xu Yan, Tang Pei
机构信息
University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.
University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.
出版信息
Methods Enzymol. 2018;603:49-66. doi: 10.1016/bs.mie.2018.01.018. Epub 2018 Mar 8.
Abstract
NMR spectroscopy is one of the major tools to provide atomic resolution protein structural information. It has been used to elucidate the molecular details of interactions between anesthetics and ion channels, to identify anesthetic binding sites, and to characterize channel dynamics and changes introduced by anesthetics. In this chapter, we present solution NMR methods essential for investigating interactions between ion channels and general anesthetics, including both volatile and intravenous anesthetics. Case studies are provided with a focus on pentameric ligand-gated ion channels and the voltage-gated sodium channel NaChBac.
摘要
核磁共振光谱是提供原子分辨率蛋白质结构信息的主要工具之一。它已被用于阐明麻醉剂与离子通道之间相互作用的分子细节，识别麻醉剂结合位点，并表征通道动力学以及麻醉剂引起的变化。在本章中，我们介绍了研究离子通道与全身麻醉剂（包括挥发性和静脉麻醉剂）之间相互作用所必需的溶液核磁共振方法。提供了案例研究，重点关注五聚体配体门控离子通道和电压门控钠通道NaChBac。
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