核磁共振作为研究膜蛋白结构、动力学和功能的一种工具。
NMR as a tool to investigate the structure, dynamics and function of membrane proteins.
作者信息
Liang Binyong, Tamm Lukas K
机构信息
Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
出版信息
Nat Struct Mol Biol. 2016 Jun 7;23(6):468-74. doi: 10.1038/nsmb.3226.
Abstract
Membrane-protein NMR occupies a unique niche for determining structures, assessing dynamics, examining folding, and studying the binding of lipids, ligands and drugs to membrane proteins. However, NMR analyses of membrane proteins also face special challenges that are not encountered with soluble proteins, including sample preparation, size limitation, spectral crowding and sparse data accumulation. This Perspective provides a snapshot of current achievements, future opportunities and possible limitations in this rapidly developing field.
摘要
膜蛋白核磁共振在确定结构、评估动力学、研究折叠以及研究脂质、配体和药物与膜蛋白的结合方面占据着独特的地位。然而，膜蛋白的核磁共振分析也面临着可溶性蛋白所没有的特殊挑战，包括样品制备、尺寸限制、谱峰拥挤和稀疏数据积累。本综述概述了这个快速发展领域的当前成就、未来机遇和可能存在的局限性。
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