Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China; Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
J Chromatogr A. 2018 Jun 1;1552:1-9. doi: 10.1016/j.chroma.2018.03.039. Epub 2018 Mar 21.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously found in the environment and have been proved to be prospectively associated with the risk of cancer. In this study, a simple method based on pipette-tip solid phase extraction (PT-SPE) and gas chromatography-mass spectrometry (GC-MS) has been firstly developed for the determination of 16 PAHs in human whole blood. Three-dimensional ionic liquid-ferrite functionalized graphene oxide nanocomposite (3D-IL-FeO-GO) was used as sorbent in PT-SPE. Compared with conventional SPE method, the PT-SPE method was solvent-saving (1.0 mL), reusable (at least 10 times) and required less blood sample (200 μL). Affecting parameters on extraction efficiency were investigated and optimized. Under the optimized conditions, a good linearity was obtained and the recoveries of 16 PAHs at three spiked levels ranged from 85.0% to 115%. The limits of quantification (LOQs) were in the range of 0.007-0.013 μg/L. Furthermore, the developed method was successfully applied to the analysis of 16 PAHs in 14 human blood samples. The results showed that the predominant PAHs in human whole blood was low-molecular-weight PAHs, with the rank order phenanthrene (PHE)> naphthalene (NAP)> fluorene (FLU)> fluoranthene (FLT)> pyrene (PYR). Because of its simplicity, accuracy and reliability, the PT-SPE method combined with GC-MS demonstrated the applicability for clinical analysis and provided more information for PAHs exposure studies.
多环芳烃（PAHs）广泛存在于环境中，已被证明与癌症风险具有潜在相关性。在这项研究中，首次建立了一种基于移液管 tips 固相萃取（PT-SPE）和气相色谱-质谱（GC-MS）的简单方法，用于测定人全血中的 16 种 PAHs。三维离子液体-铁氧体功能化氧化石墨烯纳米复合材料（3D-IL-FeO-GO）被用作 PT-SPE 中的吸附剂。与传统 SPE 方法相比，PT-SPE 方法具有节省溶剂（1.0 mL）、可重复使用（至少 10 次）和所需血样量少（200 μL）的特点。考察并优化了影响萃取效率的参数。在优化条件下，获得了良好的线性关系，16 种 PAHs 在三个加标水平的回收率在 85.0%至 115%之间。检出限（LOQs）在 0.007-0.013 μg/L 范围内。此外，该方法成功应用于 14 个人血样中 16 种 PAHs 的分析。结果表明，人全血中主要的 PAHs 是低分子量 PAHs，其顺序为菲（PHE）>萘（NAP）>芴（FLU）>荧蒽（FLT）>芘（PYR）。由于其简单、准确和可靠，GC-MS 结合的 PT-SPE 方法证明了其在临床分析中的适用性，并为 PAHs 暴露研究提供了更多信息。
