Department of Science Education, A. K. Education Faculty, Necmettin Erbakan University, Konya 42090, Turkey.
Talanta. 2018 Jul 1;184:149-155. doi: 10.1016/j.talanta.2018.02.096. Epub 2018 Feb 26.
Paper-based sensors open new avenue to produce simple, rapid, inexpensive and single-use analytical devices for a wide application fields involving medical diagnosis, food analysis and environmental monitoring. In this study, a new optical sensing platform for enantioselective recognition of chiral analytes was introduced by using in-situ synthesized silver nanoparticles-embedded transparent nanopaper. To this aim, nanopaper was obtained by environment-friendly approach using bacterial cellulose made of nanofibers and silver nanoparticles were embedded within nanopaper by an in-situ generation method. The chiral recognition capability of AgNPs was operated in both solution phase and nanopaper for the tested enantiomers. AgNPs showed a discriminative sensing response toward D-cysteine with a LOD value of 4.88 µM. The principle of optical sensing is the selective interaction of the inherently chiral AgNPs with enantiomers causing to aggregation of AgNPs to display a significant colour change from yellow to purple-brown in both aqueous phase and nanopaper. As for practical use, the obtained plasmonic nanopaper was punched into circular pieces and put on wax-printed PET film to produce disposable two-dimensional cuvette which could be inserted in an ordinary spectrophotometer. The enantiomeric percentage of D-cysteine was successfully determined by the fabricated nanopaper-based cuvettes.
基于纸张的传感器为生产用于广泛应用领域的简单、快速、廉价和一次性分析设备开辟了新途径，这些应用领域包括医疗诊断、食品分析和环境监测。在本研究中，通过使用原位合成的银纳米粒子嵌入透明纳米纸，引入了一种用于手性分析物对映选择性识别的新型光学传感平台。为此，通过使用由纳米纤维制成的细菌纤维素的环保方法获得纳米纸，并通过原位生成方法将银纳米粒子嵌入纳米纸中。在测试的对映体中，AgNPs 在溶液相和纳米纸中都表现出了手性识别能力。AgNPs 对 D-半胱氨酸表现出了有区别的传感响应，LOD 值为 4.88 µM。光学传感的原理是固有手性的 AgNPs 与对映体的选择性相互作用，导致 AgNPs 聚集，从而在水相和纳米纸中显示出从黄色到紫棕色的显著颜色变化。对于实际应用，将获得的等离子体纳米纸冲压成圆形片，并放在蜡印 PET 薄膜上，以生产可一次性使用的二维比色皿，可将其插入普通分光光度计中。通过制备的基于纳米纸的比色皿成功地确定了 D-半胱氨酸的对映体百分比。
