School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Institute of Molecular Science, Chemical Biology and Molecular Engineering, Laboratory of Education Ministry, Shanxi University, Taiyuan 030006, PR China.
Talanta. 2018 Jul 1;184:7-14. doi: 10.1016/j.talanta.2018.02.094. Epub 2018 Feb 27.
A simple Schiff base comprised of tris(2-aminoethyl)amine and salicylaldehyde was designed and synthesized by one-step reaction. Although this compound has poor selectivity for metal ions in acetonitrile, it shows high selectivity and sensitivity detection for Zn(II) ions through adjusting the solvent polarity (the volume ratio of CHCN/HO). In other words, this work provides a facile way to realize a transformation from poor to excellent feature for fluorescent probes. The bonding mode of this probe with Zn(II) ions was verified by H NMR and MS assays. The stoichiometric ratio of the probe with Zn(II) is 1:1 (mole), which matches with the Job-plot assay. The detection limitation of the probe for Zn(II) is up to 1 × 10 mol/L. The electrochemical property of the probe combined with Zn(II) was investigated by cyclic voltammetry method, and the result agreed with the theoretical calculation by the Gaussian 09 software. The probe for Zn(II) could be applied in practical samples and biological systems. The main contribution of this work lies in providing a very simple method to realize the selectivity transformation for poor selective probes. The providing way is a simple, easy and low-cost method for obtaining high selectively fluorescence probes.
一种由三（2-氨基乙基）胺和水杨醛组成的简单席夫碱通过一步反应设计并合成。尽管该化合物在乙腈中对金属离子的选择性较差，但通过调节溶剂极性（CHCN/HO 的体积比），它对 Zn(II) 离子表现出高选择性和灵敏度检测。换句话说，这项工作为实现荧光探针从差到优的特性转变提供了一种简便的方法。通过 1 H NMR 和 MS 分析验证了该探针与 Zn(II) 离子的键合模式。探针与 Zn(II)的配位比为 1:1（摩尔），与 Job-plot 分析相符。探针对 Zn(II)的检测限低至 1×10 -7 mol/L。通过循环伏安法研究了探针与 Zn(II)的电化学性质，结果与 Gaussian 09 软件的理论计算一致。该 Zn(II)探针可应用于实际样品和生物体系。这项工作的主要贡献在于提供了一种非常简单的方法来实现对选择性较差的探针的选择性转变。提供的方法是一种简单、易用且低成本的方法，用于获得高选择性的荧光探针。
