Yamashita T, Koyama J
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokkaido University.
Microbiol Immunol. 1988;32(2):199-210. doi: 10.1111/j.1348-0421.1988.tb01378.x.
In our previous paper, we reported that guinea pig splenic lymphocytes expressed two distinct Fc-receptors for homologous IgG (Fc gamma Rs), one monospecific for IgG2 (Fc gamma 2R) and the other bispecific for IgG1 and IgG2 (Fc gamma 1/gamma 2R), when analyzed by EA-rosette assay. These Fc gamma Rs on the cells were further studied by using two monoclonal antibodies toward the Fc gamma Rs on guinea pig peritoneal macrophages (anti-Fc gamma 1/gamma 2R and anti-Fc gamma 2R antibody). The anti-Fc gamma 1/gamma 2R antibody completely inhibited the rosette formation of splenic lymphocytes with IgG1-sensitized sheep erythrocytes [EA(IgG1)]. On the other hand, EA(IgG2)-rosette formation was inhibited partially by anti-Fc gamma 2R but not by anti-Fc gamma 1/gamma 2R antibody. Complete inhibition of the EA (IgG2)-rosette formation was achieved by simultaneous additions of both anti-Fc gamma 2R and anti-Fc gamma 1/gamma 2R antibodies. The binding of IgG2 antibody complexed with ovalbumin to the cells was partially inhibited by either anti-Fc gamma R antibody, and complete inhibition occurred in the presence of both the antibodies, indicating that two types of Fc gamma R, Fc gamma 1/gamma 2R, and Fc gamma 2R, are expressed on the cells. The determination of these Fc gamma Rs on B and T lymphocytes by two-color flow cytometry showed that about 52% of B lymphocytes expressed Fc gamma 1/gamma 2R alone and 32% of the cells expressed both the Fc gamma Rs. On the other hand, about 12% of T lymphocytes was found to express Fc gamma 2R alone and the cells expressing Fc gamma 1/gamma 2R were in the minority (3.8%). T lymphocytes expressing both the Fc gamma Rs were not detected. These results show that guinea pig B lymphocytes bear two types of Fc gamma Rs and are heterogeneous with regard to their Fc gamma Rs and that T lymphocytes express Fc gamma 2R mainly.
在我们之前的论文中，我们报道了通过EA-玫瑰花结试验分析时，豚鼠脾淋巴细胞表达两种不同的同源IgG Fc受体（FcγRs），一种对IgG2具有单特异性（Fcγ2R），另一种对IgG1和IgG2具有双特异性（Fcγ1/γ2R）。利用针对豚鼠腹腔巨噬细胞上FcγRs的两种单克隆抗体（抗Fcγ1/γ2R和抗Fcγ2R抗体）对细胞上的这些FcγRs进行了进一步研究。抗Fcγ1/γ2R抗体完全抑制了脾淋巴细胞与IgG1致敏绵羊红细胞[EA（IgG1）]的玫瑰花结形成。另一方面，EA（IgG2）-玫瑰花结形成被抗Fcγ2R部分抑制，但不被抗Fcγ1/γ2R抗体抑制。同时添加抗Fcγ2R和抗Fcγ1/γ2R抗体可完全抑制EA（IgG2）-玫瑰花结形成。与卵白蛋白复合的IgG2抗体与细胞的结合被任何一种抗FcγR抗体部分抑制，两种抗体同时存在时则完全抑制，表明细胞上表达两种类型的FcγR，即Fcγ1/γ2R和Fcγ2R。通过双色流式细胞术对B淋巴细胞和T淋巴细胞上这些FcγRs的测定表明，约52%的B淋巴细胞仅表达Fcγ1/γ2R，32%的细胞表达两种FcγR。另一方面，发现约12%的T淋巴细胞仅表达Fcγ2R，表达Fcγ1/γ2R的细胞占少数（3.8%）。未检测到同时表达两种FcγR的T淋巴细胞。这些结果表明，豚鼠B淋巴细胞带有两种类型的FcγR，并且在FcγR方面是异质的，而T淋巴细胞主要表达Fcγ2R。
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