褪黑素和 MTNR1B 变体与古吉拉特邦人群 2 型糖尿病的关联。
Association of melatonin &MTNR1B variants with type 2 diabetes in Gujarat population.
机构信息
Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, Gujarat, India.
Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, 390002, Gujarat, India.
出版信息
Biomed Pharmacother. 2018 Jul;103:429-434. doi: 10.1016/j.biopha.2018.04.058. Epub 2018 Apr 24.
AIM/HYPOTHESIS: Melatonin is a circadian rhythm regulator and any imbalance in its levels can be related to various metabolic disorders. Melatonin and the genetic variants of Melatonin Receptor 1B (MTNR1B) are reported to be associated with Type 2 Diabetes (T2D) susceptibility. The aim of the present study was to investigate i) plasma melatonin levels ii) Single Nucleotide Polymorphisms (SNPs) of MTNR1B and iii) Genotype-phenotype correlation analysis in T2D patients.
METHODS
Plasma and PBMCs were separated from venous blood of 478 diabetes patients and 502 controls. Genomic DNA was isolated from PBMCs. PCR-RFLP was used for genotyping. Melatonin was estimated from plasma samples by ELISA.
RESULTS
Our study suggests: i) decreased plasma melatonin levels in T2D patients and, ii) association of MTNR1B rs10830963 GG genotype with increased Fasting Blood Glucose (FBG).
CONCLUSION
It can be concluded that reduced titer of melatonin along with altered FBG due to MTNR1B genetic variant could act as a potent risk factor towards T2D in Gujarat population.
目的/假设：褪黑素是一种昼夜节律调节剂，其水平的任何失衡都可能与各种代谢紊乱有关。褪黑素和褪黑素受体 1B（MTNR1B）的遗传变异与 2 型糖尿病（T2D）易感性有关。本研究的目的是研究 i）血浆褪黑素水平 ii）MTNR1B 的单核苷酸多态性（SNP）和 iii）T2D 患者的基因型-表型相关性分析。
方法
从 478 名糖尿病患者和 502 名对照者的静脉血中分离血浆和 PBMCs。从 PBMCs 中分离基因组 DNA。PCR-RFLP 用于基因分型。通过 ELISA 从血浆样本中估计褪黑素。
结果
我们的研究表明：i）T2D 患者血浆褪黑素水平降低，ii）MTNR1B rs10830963 GG 基因型与空腹血糖（FBG）升高相关。
结论
可以得出结论，由于 MTNR1B 遗传变异导致的褪黑素滴度降低和 FBG 改变可能是古吉拉特邦人群 T2D 的一个潜在危险因素。
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