Guerin G, Varewyck H, Bertaud M, Chasset P
Laboratoire de Génétique Biochimique, INRA-CRJ, Jouy-en-Josas, France.
Anim Genet. 1988;19(1):1-9. doi: 10.1111/j.1365-2052.1988.tb00782.x.
A horse family in which a recombination occurred in the chromosome region coding for the serological specificities of the ELA complex and those of the A blood group system of a mare was further analysed by mixed lymphocyte reaction (MLR) and Southern blot hybridization. This family consisted of a stallion, a mare and five full sibs. The stallion and the mare were heterozygous for internationally recognized ELA specificities while only the mare was heterozygous for the A blood group system. MLR between all members of the family confirmed that the stallion possessed two different ELA haplotypes and suggested that recombination in the mare occurred outside the segment delimited by the ELA-A locus and the MLR region. DNA samples from all individuals were investigated by Southern blot analysis using three restriction enzymes (EcoRI, HindIII or TaqI), three human HLA probes (one of class I cDNA and two of class II probes), one cDNA (DR beta) and one genomic (DQ alpha). Class I and class II restriction fragments of the mare segregated in accordance to the ELA specificities and thus clearly confirming that the crossing-over did not occur between the ELA-A gene and the class I, class II region nor between DR beta and DQ alpha subsets. The A blood group genetic determinants would thus be situated outside the ELA region defined by class I and class II genes.
对一个马家族进行了进一步分析，该家族中一匹母马的编码ELA复合体血清学特异性和A血型系统血清学特异性的染色体区域发生了重组，采用混合淋巴细胞反应（MLR）和Southern印迹杂交法进行分析。这个家族由一匹种马、一匹母马和五个全同胞组成。种马和母马对于国际公认的ELA特异性是杂合的，而只有母马对于A血型系统是杂合的。家族所有成员之间的MLR证实种马拥有两种不同的ELA单倍型，并表明母马中的重组发生在由ELA - A基因座和MLR区域界定的片段之外。使用三种限制性内切酶（EcoRI、HindIII或TaqI）、三种人类HLA探针（一种I类cDNA探针和两种II类探针）、一种cDNA（DRβ）和一种基因组（DQα），通过Southern印迹分析对所有个体的DNA样本进行了研究。母马的I类和II类限制性片段根据ELA特异性进行分离，从而清楚地证实了在ELA - A基因与I类、II类区域之间以及DRβ和DQα亚组之间没有发生交换。因此，A血型遗传决定因素将位于由I类和II类基因定义的ELA区域之外。
