Simmons K W, Wooley R E, Brown J
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Appl Environ Microbiol. 1988 Mar;54(3):760-7. doi: 10.1128/aem.54.3.760-767.1988.
The antibiotic resistance and virulence profiles of Salmonella spp. isolated from healthy (group 1) and ill (group 2) swine were compared. Parameters studied included colicin and siderophore production; mannose-sensitive hemagglutination of erythrocytes; resistance to the lethal effect of serum complement; resistance to antibiotics; and the transmissibility of these characteristics to recipient organisms. Group 1 (19 isolates) had 14 serotypes, and group 2 (20 isolates) had 2 serotypes. Isolates from group 2 were resistant to more antibiotics and had a greater ability to hemagglutinate erythrocytes and transfer R plasmids to recipient organisms, but a lesser ability to produce siderophore than group 1. All 39 isolates resisted the lethal effects of serum complement. Colicin was produced by 1 of 19 from group 1 and 0 of 20 from group 2. A donor Escherichia coli isolated from a pig with enteritis transferred R plasmids to 62% of group 1 and 0% of group 2 Salmonella spp. when they were used as recipient organisms. A transconjugant from the mating of donor E. coli to a group 1 Salmonella spp. was further able to pass an R plasmid to recipient E. coli and salmonellae. Plasmid isolation from group 1 yielded 1 of 19 strains with a 56-megadalton plasmid, while 20 of 20 strains from group 2 contained three to five plasmids from 2.4 to 60 megadaltons in size.
比较了从健康猪（第1组）和患病猪（第2组）中分离出的沙门氏菌属的抗生素耐药性和毒力特征。研究的参数包括大肠杆菌素和铁载体的产生；红细胞的甘露糖敏感血凝作用；对血清补体致死作用的抗性；对抗生素的抗性；以及这些特征向受体生物体的传递性。第1组（19株分离株）有14种血清型，第2组（20株分离株）有2种血清型。第2组的分离株对更多抗生素具有抗性，红细胞血凝能力和将R质粒转移至受体生物体的能力更强，但与第1组相比，产生铁载体的能力较弱。所有39株分离株均能抵抗血清补体的致死作用。第1组19株中有1株产生大肠杆菌素，第2组20株中无1株产生。从患肠炎的猪中分离出的供体大肠杆菌将R质粒转移至62%的第1组沙门氏菌属，而作为受体生物体时，转移至第2组沙门氏菌属的比例为0%。供体大肠杆菌与第1组沙门氏菌属交配产生的转接合子能够进一步将R质粒传递给受体大肠杆菌和沙门氏菌。从第1组分离质粒，19株中有1株获得了一个56兆道尔顿的质粒，而第2组20株中有20株含有三至五个大小在2.4至60兆道尔顿之间的质粒。
