Kusnecov A W, Husband A J, King M G, Pang G, Smith R
Faculty of Medicine, University of Newcastle, New South Wales, Australia.
Brain Behav Immun. 1987 Mar;1(1):88-97. doi: 10.1016/0889-1591(87)90010-9.
Experiments were undertaken in rats to investigate the effects of in vivo infusion of beta-endorphin (BEP) on subsequent Con A-induced proliferation and interleukin 2 (IL-2) production by spleen cells in vitro. BEP administration induced a dose-dependent enhancement of the proliferative response to Con A. Infusion of the opiate antagonist naloxone (NAL) inhibited the Con A response and infusion of NAL prior to BEP resulted in even further inhibition. None of these treatments resulted in detectable alterations in IL-2 production after 48 h in culture. To demonstrate a direct interaction between BEP and lymphocytes, spleen cells were incubated in vitro with varying concentrations of BEP and/or NAL. Enhanced Con A-induced proliferation was observed following incubation with BEP in the range 10(-12) to 10(-9) M (levels comparable to the effective in vivo doses) and this effect was abrogated by NAL pretreatment (10(-6) M). These data indicate a role for BEP in enhancing lymphocyte reactivity which is to some extent dependent on opiate receptors on the cell surface. This report extends the evidence obtained from in vitro experiments implicating endogenous opioids in modulation of host immunity by demonstrating that these effects can be obtained in vivo.
在大鼠身上进行了实验，以研究体内输注β-内啡肽（BEP）对随后体外脾细胞经刀豆蛋白A（Con A）诱导的增殖以及白细胞介素2（IL-2）产生的影响。给予BEP可诱导对Con A的增殖反应呈剂量依赖性增强。输注阿片拮抗剂纳洛酮（NAL）可抑制对Con A的反应，且在给予BEP之前输注NAL会导致更显著的抑制。在培养48小时后，这些处理均未导致IL-2产生出现可检测到的改变。为了证明BEP与淋巴细胞之间的直接相互作用，将脾细胞与不同浓度的BEP和/或NAL在体外进行孵育。在与10(-12)至10(-9) M范围内的BEP孵育后（该水平与有效的体内剂量相当），观察到Con A诱导的增殖增强，且这种效应可被NAL预处理（10(-6) M）消除。这些数据表明BEP在增强淋巴细胞反应性方面发挥作用，这在一定程度上依赖于细胞表面的阿片受体。本报告通过证明这些效应可在体内获得，扩展了从体外实验中获得的有关内源性阿片类物质参与调节宿主免疫的证据。
