Department of Pharmacognosy, Faculty of Pharmacy, Near East University, Nicosia, Mersin 10, Turkey.
Department of Bioengineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Urla-Izmir, Turkey.
Phytochemistry. 2018 Jul;151:91-98. doi: 10.1016/j.phytochem.2018.04.006. Epub 2018 Apr 17.
The microbial transformation of cyclocanthogenol (CCG), Astragalus sp. originated sapogenin, by the endophytic fungus Alternaria eureka 1E1BL1 isolated from Astragalus angustifolius was investigated. Hydroxylation, oxidation, epoxidation, O-methylation, ring-expansion and methyl migration reactions were observed on the triterpenoid skeleton. As a result, eight metabolites were isolated and the structures of the previously undescribed compounds were established by 1-D, 2-D NMR and HR-MS analyses.
从窄叶黄花 （Astragalus angustifolius） 内生真菌棘孢木霉（Alternaria eureka 1E1BL1）中分离得到的内生真菌，对环黄芪醇（CCG）、黄芪属植物来源的甾体皂甙元进行了微生物转化研究。在三萜骨架上观察到了羟基化、氧化、环氧化、O-甲基化、环扩张和甲基迁移反应。结果分离得到 8 种代谢产物，通过 1D、2D NMR 和 HR-MS 分析确定了以前未描述的化合物的结构。
