Van Lier R A, Pool M O, Kabel P, Mous S, Terpstra F, De Rie M A, Melief C J, Miedema F
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Eur J Immunol. 1988 May;18(5):811-6. doi: 10.1002/eji.1830180523.
Anti-CD27 monoclonal antibodies react with a cell surface molecule expressed on medullary thymocytes and a large subpopulation (75%) of peripheral blood T lymphocytes. This study was undertaken to analyze the functional capacities of CD27+ and CD27- subpopulations within the CD4+ subset. In addition, we investigated whether CD27 subpopulations belong to two mutually exclusive T cell sublineages. Proliferation upon lectin stimulation by either phytohemagglutinin or pokeweed mitogen (PWM) was found to be consistently higher in CD4+CD27+ cells compared to CD4+CD27- cells. In contrast, CD27+ and CD27- cells did not differ in anti-CD3, soluble antigen or interleukin (IL)2-induced proliferation. In PWM-driven B cell differentiation, CD4+CD27+ cells provided helper activity on IgM production, while CD4+CD27- cells did not. The differences observed between CD4+CD27+ and CD4+CD27- cells in both proliferation and T helper activity on IgM production can, at least in part, be explained by the inadequate production of IL2 by CD27- cells upon lectin stimulation. In contrast, CD27+ and CD27- subpopulations did not differ in the production of interferon-gamma. CD27- cells could be induced to express the CD27 antigen through stimulation of these cells with immobilized anti-CD3 antibodies. After 3 days of culture, approximately 50% of the cells had CD27 membrane expression, whereas after 6 days 80% of the cells express the antigen. We conclude that anti-CD27 antibodies identify two functionally distinct T cell populations within the CD4+ subset. These subpopulations apparently do not belong to two separate T cell sublineages, but may reflect differences in the activation state of CD27+ vs. CD27- peripheral blood T lymphocytes.
抗CD27单克隆抗体可与髓质胸腺细胞以及外周血T淋巴细胞的一个大的亚群（75%）上表达的细胞表面分子发生反应。本研究旨在分析CD4⁺亚群中CD27⁺和CD27⁻亚群的功能能力。此外，我们还研究了CD27亚群是否属于两个相互排斥的T细胞亚系。发现与CD4⁺CD27⁻细胞相比，CD4⁺CD27⁺细胞在受到植物血凝素或商陆丝裂原（PWM）的凝集素刺激后的增殖能力一直更高。相比之下，CD27⁺和CD27⁻细胞在抗CD3、可溶性抗原或白细胞介素（IL）2诱导的增殖方面没有差异。在PWM驱动的B细胞分化过程中，CD4⁺CD27⁺细胞对IgM产生具有辅助活性，而CD4⁺CD27⁻细胞则没有。在增殖和对IgM产生的T辅助活性方面，CD4⁺CD27⁺和CD4⁺CD27⁻细胞之间观察到的差异至少部分可以通过CD27⁻细胞在凝集素刺激后IL2产生不足来解释。相比之下，CD27⁺和CD27⁻亚群在干扰素-γ的产生方面没有差异。通过用固定化抗CD3抗体刺激这些细胞，可以诱导CD27⁻细胞表达CD27抗原。培养3天后，约50%的细胞有CD27膜表达，而培养6天后，80%的细胞表达该抗原。我们得出结论，抗CD27抗体可识别CD4⁺亚群中两个功能不同的T细胞群体。这些亚群显然不属于两个独立的T细胞亚系，而是可能反映了CD27⁺与CD27⁻外周血T淋巴细胞激活状态的差异。
