Harada Sohei, Ishii Yoshikazu, Saga Tomoo, Aoki Kotaro, Tateda Kazuhiro
Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540, Tokyo, Japan; Department of Infectious Diseases, Cancer Institute Hospital, Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto-ku 135-8550, Tokyo, Japan.
Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540, Tokyo, Japan.
Diagn Microbiol Infect Dis. 2018 Aug;91(4):354-359. doi: 10.1016/j.diagmicrobio.2018.03.010. Epub 2018 Mar 20.
Although severe infections caused by hypervirulent Klebsiella pneumoniae isolates, such as K1 isolates belonging to sequence type (ST) 23, have been a significant problem in Asian countries, epidemiology of these isolates in Japan remains unclear. We performed a nationwide molecular epidemiological study of K. pneumoniae K1 and K2 isolates in Japan. Of the 259K. pneumoniae isolates collected, 14 and 16 isolates were identified as capsular genotypes K1 and K2, respectively. All K1 isolates were ST23 or its closely related clones and showed high genetic similarity by pulsed-field gel electrophoresis (PFGE) and the DiversiLab system (DL). K2 isolates, belonging to ST14, ST25, ST65, ST86, and ST110, were more genetically diverse than K1 isolates. Isolates belonging to a specific ST showed identical virulence gene profiles with a few exceptions. PFGE and DL results using K1 and K2 isolates were generally in agreement.
尽管高毒力肺炎克雷伯菌分离株（如属于序列型（ST）23的K1分离株）引起的严重感染在亚洲国家一直是一个重大问题，但这些分离株在日本的流行病学情况仍不清楚。我们在日本开展了一项关于肺炎克雷伯菌K1和K2分离株的全国性分子流行病学研究。在收集的259株肺炎克雷伯菌分离株中，分别有14株和16株被鉴定为荚膜基因型K1和K2。所有K1分离株均为ST23或其密切相关克隆，通过脉冲场凝胶电泳（PFGE）和DiversiLab系统（DL）显示出高度的遗传相似性。属于ST14、ST25、ST65、ST86和ST110的K2分离株在遗传上比K1分离株更为多样。属于特定ST的分离株显示出相同的毒力基因谱，仅有少数例外。使用K1和K2分离株的PFGE和DL结果总体一致。
