使用环形间隙射频线圈在15T下对离体小鼠大脑进行显微镜检查。
Ex vivo mouse brain microscopy at 15T with loop-gap RF coil.
作者信息
Cohen Ouri, Ackerman Jerome L
机构信息
Athinoula A. Martinos Center for Biomedical Imaging Department of Imaging, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA; Department of Radiology, Harvard Medical School, Boston, MA 02115, USA.
Athinoula A. Martinos Center for Biomedical Imaging Department of Imaging, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA; Department of Radiology, Harvard Medical School, Boston, MA 02115, USA.
出版信息
Magn Reson Imaging. 2018 Sep;51:1-6. doi: 10.1016/j.mri.2018.04.010. Epub 2018 Apr 18.
The design of a loop-gap-resonator RF coil optimized for ex vivo mouse brain microscopy at ultra high fields is described and its properties characterized using simulations, phantoms and experimental scans of mouse brains fixed in 10% formalin containing 4 mM Magnevist™. The RF (B) and magnetic field (B) homogeneities are experimentally quantified and compared to electromagnetic simulations of the coil. The coil's performance is also compared to a similarly sized surface coil and found to yield double the sensitivity. A three-dimensional gradient-echo (GRE) sequence is used to acquire high resolution mouse brain scans at (47 μm) resolution in 1.8 h and a 20 × 20 × 19 μm resolution in 27 h. The high resolution obtained permitted clear visualization and identification of multiple structures in the ex vivo mouse brain and represents, to our knowledge, the highest resolution ever achieved for a whole mouse brain. Importantly, the coil design is simple and easy to construct.
本文描述了一种为超高场下离体小鼠脑显微镜检查优化的环形间隙谐振器射频线圈的设计，并通过模拟、体模以及对固定于含4 mM马根维显™的10%福尔马林中的小鼠脑进行实验扫描来表征其特性。通过实验对射频（B）和磁场（B）均匀性进行了量化，并与线圈的电磁模拟结果进行了比较。还将该线圈的性能与尺寸类似的表面线圈进行了比较，发现其灵敏度提高了一倍。使用三维梯度回波（GRE）序列，在1.8小时内以（47μm）分辨率、在27小时内以20×20×19μm分辨率获取了高分辨率小鼠脑扫描图像。所获得的高分辨率使得能够清晰地可视化和识别离体小鼠脑中的多个结构，据我们所知，这代表了对整个小鼠脑所实现的最高分辨率。重要的是，该线圈设计简单且易于构建。
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