Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Institute of Plant Protection, Guizhou Academy of Agricultural Sciences, Guiyang 550009, China.
Gene. 2018 Jul 15;663:131-137. doi: 10.1016/j.gene.2018.04.031. Epub 2018 Apr 19.
Pesticides have been used extensively for pest control, resulting in serious pesticide resistance. It is extremely valuable to identify the resistance genes related to pest control. In our previous studies, we reported that Ran is the deltamethrin (DM) resistance-associated gene. To clarify whether Ran is also related to the resistance of other pesticides, we selected four kinds of pesticides, including parathion, DDT, carbaryl and pleocidin, for further study. The results showed that Ran could be up-regulated by all pesticides. We further verified the relationship between Ran and resistance to the 4 pesticides by Ran RNAi, Ran overexpression and cell apoptosis. We found that the dsRNA of Ran induced more cell apoptosis than the control. Ran overexpression can significantly improve cell tolerance to various pesticides. These results demonstrate that Ran is associated with the resistance to and tolerance of multiple pesticides. Our evidence suggests the Ran is a potential molecular target gene of resistance control.
杀虫剂被广泛用于害虫防治，导致严重的抗药性。鉴定与害虫防治相关的抗性基因非常有价值。在我们之前的研究中，我们报道 Ran 是拟除虫菊酯（DM）抗性相关基因。为了阐明 Ran 是否也与其他杀虫剂的抗性有关，我们选择了四种杀虫剂，包括对硫磷、DDT、西维因和 pleocidin，进行进一步研究。结果表明，Ran 可以被所有杀虫剂上调。我们通过 Ran RNAi、Ran 过表达和细胞凋亡进一步验证了 Ran 与 4 种杀虫剂抗性的关系。我们发现 Ran 的 dsRNA 诱导的细胞凋亡比对照多。Ran 过表达可以显著提高细胞对各种杀虫剂的耐受性。这些结果表明，Ran 与多种杀虫剂的抗性和耐受性有关。我们的证据表明，Ran 是抗性控制的一个潜在的分子靶标基因。
