Zhang J S, Xia Y T, Zheng R C, Liang Z Y, Shen Y J, Li Y F, Nie M, Gu C, Wang H
Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Vet J. 2018 Apr;234:24-26. doi: 10.1016/j.tvjl.2018.01.007. Epub 2018 Feb 2.
Thirty clinical isolates of H. parasuis from pig farms in eastern China were screened for antimicrobial susceptibility. A novel plasmid, designated pHPSGC, was extracted from one isolate with evidence of resistance (elevated minimum inhibitory concentration) to florfenicol. DNA sequencing demonstrated that pHPSGC (5297 base pairs) contains three open reading frames (ORFs), corresponding to the genes rep, floR and lysR. The rep gene of pHPSGC shared 99% sequence identity with the rep gene of pHPS1019. In addition, the region containing floR and lysR in pHPSGC shared 99% similarity with the corresponding region of pCCK381. pHPSGC may be derived from a recombination event between pHPS1019 and pCCK381. A florfenicol resistance gene in H. parasuis may have been transferred via recombination between different plasmids.
对来自中国东部猪场的30株副猪嗜血杆菌临床分离株进行了药敏试验。从一株对氟苯尼考耐药（最低抑菌浓度升高）的分离株中提取了一种新的质粒，命名为pHPSGC。DNA测序表明，pHPSGC（5297个碱基对）包含三个开放阅读框（ORF），分别对应rep、floR和lysR基因。pHPSGC的rep基因与pHPS1019的rep基因序列一致性为99%。此外，pHPSGC中包含floR和lysR的区域与pCCK381的相应区域相似度为99%。pHPSGC可能来源于pHPS1019和pCCK381之间的重组事件。副猪嗜血杆菌中的氟苯尼考耐药基因可能通过不同质粒之间的重组进行了转移。
