超声对多酶体系中半乳糖寡糖和葡萄糖酸生产的影响。
Impact of ultrasound on galactooligosaccharides and gluconic acid production throughout a multienzymatic system.
机构信息
Departamento de Ingeniería Química y Ambiental, Facultad de Ingeniería, Universidad Nacional de Colombia Sede Bogotá, Carrera 30 N° 45-03, Bogotá, Colombia.
Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM) CEI (CSIC+UAM), Nicolás Cabrera, 9. Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
出版信息
Ultrason Sonochem. 2018 Jun;44:177-183. doi: 10.1016/j.ultsonch.2018.02.022. Epub 2018 Feb 12.
Galactooligosaccharides (GOS), recognised prebiotic, can be industrially produced from lactose and commercial β-galactosidase (β-gal) from Kluyveromyces lactis. Residual lactose and glucose limit GOS applications. To handle this problem, a multienzymatic system, with β-gal and glucose oxidase (Gox), was proposed to reduce glucose content in reaction media through its oxidation to gluconic acid (GA). Besides, ultrasound (US) probe effect over the multienzymatic system to produce GOS and GA has been evaluated. A production around 40% of GOS was found in all treatments after the first hour of reaction. However, glucose consumption and GA production was significantly higher (P < 0.05) for sequential reaction assisted by US, obtaining the best production of GOS (49%) and GA (28%) after 2 h of reaction. The conformational and residual activity changes of enzymes under US conditions were also evaluated, Gox being positively affected whereas in β-gal hardly any change was found.
半乳糖寡糖（GOS）是一种公认的益生元，可以从乳糖和商业来源的乳糖酶（β-半乳糖苷酶，β-gal）生产。残留的乳糖和葡萄糖限制了 GOS 的应用。为了解决这个问题，提出了一种多酶系统，利用β-半乳糖苷酶和葡萄糖氧化酶（Gox），通过氧化将葡萄糖转化为葡萄糖酸（GA），从而降低反应介质中的葡萄糖含量。此外，还评估了超声（US）探头对生产 GOS 和 GA 的多酶系统的影响。反应开始后 1 小时，所有处理组的 GOS 产量均达到 40%左右。然而，在 US 辅助的顺序反应中，葡萄糖的消耗和 GA 的产生明显更高（P<0.05），反应 2 小时后获得了最高的 GOS（49%）和 GA（28%）产量。还评估了酶在 US 条件下的构象和残余活性变化，发现 Gox 受到积极影响，而β-半乳糖苷酶几乎没有变化。
Zotero 插件