Dieriks Birger Victor, Dean Justin M, Aronica Eleonora, Waldvogel Henry J, Faull Richard L M, Curtis Maurice A
Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Auckland, New Zealand.
Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Auckland, New Zealand.
Dev Neurosci. 2018;40(2):145-161. doi: 10.1159/000487633. Epub 2018 Apr 20.
Fatty acid-binding proteins (FABPs) are a family of transport proteins that facilitate intracellular transport of fatty acids. Despite abundant expression in the brain, the role that FABPs play in the process of cell proliferation and migration in the subventricular zone (SVZ) remains unclear. Our results provide a detailed characterisation of FABP3, 5, and 7 expression in adult and fetal human and sheep SVZ. High FABP5 expression was specifically observed in the adult human SVZ and co-labelled with polysialylated neural cell adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP), GFAPδ, and proliferating cell nuclear antigen (PCNA), indicating a role for FABP5 throughout the full maturation process of astrocytes and neuroblasts. Some FABP5+ cells had a radial glial-like appearance and co-labelled with the radial glia markers vimentin (40E-C) and GFAP. In the fetal human brain, FABP5 was expressed by radial glia cells throughout the ventricular zone. In contrast, radial glia-like cells in sheep highly expressed FABP3. Taken together, these differences highlight the species-specific expression profile of FABPs in the SVZ. In this study, we demonstrate the distribution of FABP in the adult human SVZ and fetal ventricular zone and reveal its expression on persistent radial glia that may be involved in adult neurogenesis.
脂肪酸结合蛋白（FABPs）是一类促进脂肪酸细胞内运输的转运蛋白。尽管在大脑中大量表达，但FABPs在脑室下区（SVZ）细胞增殖和迁移过程中所起的作用仍不清楚。我们的研究结果详细描述了FABP3、5和7在成人及胎儿人类和绵羊SVZ中的表达情况。在成人人类SVZ中特别观察到高FABP5表达，并与多唾液酸神经细胞粘附分子（PSA-NCAM）、胶质纤维酸性蛋白（GFAP）、GFAPδ和增殖细胞核抗原（PCNA）共标记，表明FABP5在星形胶质细胞和成神经细胞的整个成熟过程中发挥作用。一些FABP5+细胞具有放射状胶质样外观，并与放射状胶质标记物波形蛋白（40E-C）和GFAP共标记。在胎儿人类大脑中，FABP5在整个脑室区的放射状胶质细胞中表达。相比之下, 绵羊的放射状胶质样细胞高度表达FABP3。综上所述，这些差异突出了FABPs在SVZ中的物种特异性表达谱。在本研究中，我们展示了FABP在成人人类SVZ和胎儿脑室区的分布，并揭示了其在可能参与成体神经发生的持续放射状胶质细胞上的表达。
