Biological Processes Laboratory (LPB), São Carlos School of Engineering (EESC), University of São Paulo (USP) , São Carlos , SP , Brazil.
Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP , Campinas , SP , Brasil.
Environ Technol. 2019 Oct;40(24):3216-3226. doi: 10.1080/09593330.2018.1468491. Epub 2018 Apr 26.
Phenol removal was investigated in anaerobic fixed-structured bed reactors, namely R1 and R2, treating synthetic wastewater simulating the soluble fraction of vinasse under strictly methanogenic (R1) and simultaneous methanogenic/sulfidogenic conditions (R2). Next-generation sequencing (Illumina MiSeq System) was used to further characterize the microbial communities in both systems. Phenol was completely and stably removed in R1 after a short operating period (≈55 days). Conversely, phenol removal in R2 required a longer period for biomass acclimation (≈125 days) to reach levels equivalent to R1. Volatile fatty acids (VFA) accumulation in R2, mainly due to the inhibition of the acetoclastic methanogenesis by sulfide, may have limited phenol removal in the initial operating phases, as intermediate steps from phenol degradation are thermodynamically dependent on the removal of acetate, hydrogen and bicarbonate. Overall, the potential for anaerobically removing phenol from complex wastewaters was confirmed, even at low phenol/COD ratios. 16S rRNA gene sequencing analysis showed a high correlation of taxonomic profile between R1 and the inoculum, whereas a lower correlation was observed between R2 and the inoculum samples. Functional inference further indicated that and genera in R1 and genus in both reactors possibly played a key-role in phenol degradation.
采用严格产甲烷条件（R1）和同时产甲烷/产硫化物条件（R2）的厌氧固定床反应器（R1 和 R2）处理模拟酒糟可溶部分的合成废水，考察了酚的去除情况。采用下一代测序（Illumina MiSeq 系统）进一步对两个系统中的微生物群落进行了特征描述。在短的运行期（约 55 天）后，R1 中酚可完全且稳定去除。相反，R2 中酚的去除需要更长的生物驯化期（约 125 天）才能达到与 R1 相当的水平。R2 中挥发性脂肪酸（VFA）的积累，主要是由于硫化物对乙酰化产甲烷的抑制，可能在初始运行阶段限制了酚的去除，因为酚降解的中间步骤在热力学上取决于乙酸盐、氢和碳酸氢盐的去除。总的来说，即使在低酚/COD 比的情况下，也证实了从复杂废水中厌氧去除酚的潜力。16S rRNA 基因测序分析表明，R1 与接种物之间的分类图谱高度相关，而 R2 与接种物样本之间的相关性较低。功能推断进一步表明，R1 中的 和 属和两个反应器中的 属可能在酚降解中起关键作用。
