Liao Yuqian, Liao Yulu, Li Jun, Liu Liyan, Li Junyu, Wan Yiye, Peng Lixiang
1 Department of Medical Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi Province, China.
2 Department of Radiation Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi Province, China.
Int J Biol Markers. 2018 Aug;33(3):301-307. doi: 10.1177/1724600818754752. Epub 2018 Apr 23.
Polymorphisms in miRNA machinery genes have been proved to be related to risk or survival of several kinds of cancers, but the results are controversial and the role of these polymorphisms in gastric cancer remains uncertain. In our study, we investigated the association between five genetic variants in miRNA machinery genes ( DICER, RAN, XPO5 [name of the gene]) and clinical outcomes in Chinese gastric cancer patients.
A total of 96 patients with stage IB-III gastric cancer treated with radical gastrectomy and adjuvant chemotherapy of oxaliplatin and fluorouracils were analyzed. The MassARRAY MALDI-TOF system was used to determine the genotypes.
DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers. After adjustment by age, sex, differentiation, T stage, and lymph node status, XPO5 rs2257082 CC genotype carriers were found to have worse disease-free survival than CT+TT genotype carriers (adjusted HR 3.099; 95% CI 1.270, 7.564; P=0.013), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048).
These results indicated that genetic variants in miRNA machinery genes might be associated with the clinicopathological features and prognosis of completely resected gastric cancer patients.
已证实微小RNA（miRNA）机制基因的多态性与几种癌症的风险或生存率相关，但结果存在争议，这些多态性在胃癌中的作用仍不确定。在我们的研究中，我们调查了miRNA机制基因（DICER、RAN、XPO5 [基因名称]）中的五个基因变异与中国胃癌患者临床结局之间的关联。
分析了总共96例接受根治性胃切除术及奥沙利铂和氟尿嘧啶辅助化疗的IB - III期胃癌患者。采用MassARRAY MALDI - TOF系统确定基因型。
与AA基因型相比，DICER rs3742330 AG + GG基因型与更晚期的T分期相关（P = 0.009）。与CT + TT基因型携带者相比，XPO5 rs2257082 CC基因型的患者中低分化肿瘤更多。在按年龄、性别、分化程度、T分期和淋巴结状态进行调整后，发现XPO5 rs2257082 CC基因型携带者的无病生存率比CT + TT基因型携带者差（调整后风险比3.099；95%置信区间1.270, 7.564；P = 0.013），RAN rs14035 CC基因型携带者的三年总生存率高于CT + TT基因型携带者（调整后风险比3.174；95%置信区间1.010, 9.973；P = 0.048）。
这些结果表明，miRNA机制基因中的基因变异可能与完全切除的胃癌患者的临床病理特征和预后相关。
