Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan.
Fish Shellfish Immunol. 2018 Jul;78:109-113. doi: 10.1016/j.fsi.2018.04.036. Epub 2018 Apr 21.
Shrimp, as invertebrates, have an open vasculature that allows circulating hemocytes to infiltrate the tissues, where they are referred to as sessile hemocytes. Sessile hemocytes are known to express immune-related genes, but it is not known whether their functions differ from those of circulating hemocytes. To answer this question, we enriched them from suspensions of different tissues using discontinuous density gradient centrifugation and analyzed their transcripts by RNA-seq. The results suggest that circulating hemocytes and sessile hemocytes of the gills are in a state that could react quickly to pathogens, immune-related genes expression of sessile hemocytes differ from circulating hemocytes, and the gills, heart and lymphoid organs have cells that express immune-related genes that are different from hemocytes.
虾类作为无脊椎动物，其开放式脉管系统允许循环血细胞渗透到组织中，在组织中被称为固定血细胞。已知固定血细胞表达与免疫相关的基因，但尚不清楚它们的功能是否与循环血细胞不同。为了回答这个问题，我们使用不连续密度梯度离心法从不同组织的悬浮液中富集它们，并通过 RNA-seq 分析它们的转录本。结果表明，鳃的循环血细胞和固定血细胞处于能够快速应对病原体的状态，固定血细胞的免疫相关基因表达与循环血细胞不同，鳃、心脏和淋巴器官具有表达与血细胞不同的免疫相关基因的细胞。
