Collins Chelsea, Almuzara Marisa, Saigo Mariana, Montaña Sabrina, Chiem Kevin, Traglia German, Mussi Maria Alejandra, Tolmasky Marcelo, Iriarte Andres, Vay Carlos, Ramirez Maria Soledad
Department of Biological Science, California State University Fullerton, 800 N State College Blvd, Fullerton, CA, 92831, USA.
Laboratorio de Bacteriología Clínica, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina.
Curr Microbiol. 2018 Aug;75(8):1084-1089. doi: 10.1007/s00284-018-1498-9. Epub 2018 Apr 23.
The spread of antibiotic resistance is rapidly threatening the effectiveness of antibiotics in the clinical setting. Many infections are being caused by known and unknown pathogenic bacteria that are resistant to many or all antibiotics currently available. Empedobacter falsenii is a nosocomial pathogen that can cause human infections. E. falsenii Wf282 strain was found to be resistant to many antibiotics, including carbapenems and colistin. Whole-genome shotgun sequencing of the strain was performed, and distinct features were identified. A novel metallo-β-lactamase, named EBR-2, was found, suggesting a potential role of E. falsenii as a reservoir of β-lactamases and other resistance determinants also found in its genome. The EBR-2 protein showed the highest catalytic efficiency for penicillin G as compared to meropenem and ampicillin and was unable to hydrolyze cefepime. The results described in this work broaden the current understanding of the role of β-lactamases in the Flavobacteriaceae family and suggest that E. falsenii Wf282 may be a reservoir of these novel resistance determinants.
抗生素耐药性的传播正在迅速威胁抗生素在临床环境中的有效性。许多感染是由已知和未知的病原菌引起的，这些病原菌对目前可用的许多或所有抗生素都具有耐药性。假弗氏埃希菌是一种可导致人类感染的医院病原菌。发现假弗氏埃希菌Wf282菌株对包括碳青霉烯类和黏菌素在内的许多抗生素具有耐药性。对该菌株进行了全基因组鸟枪法测序，并鉴定出了独特的特征。发现了一种名为EBR-2的新型金属β-内酰胺酶，这表明假弗氏埃希菌可能作为β-内酰胺酶和其他耐药决定因素的储存库，这些也在其基因组中被发现。与美罗培南和氨苄西林相比，EBR-2蛋白对青霉素G显示出最高的催化效率，并且无法水解头孢吡肟。这项工作中描述的结果拓宽了目前对β-内酰胺酶在黄杆菌科中作用的理解，并表明假弗氏埃希菌Wf282可能是这些新型耐药决定因素的储存库。
