Department of Cardiothoracic Surgery, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China.
Eur Rev Med Pharmacol Sci. 2018 Apr;22(7):1929-1936. doi: 10.26355/eurrev_201804_14717.
It is to study the stimulation and possible active mechanism of miRNA-21 on AGS proliferation of gastric cancer.
AGS gastric cancer cells were cultivated in vitro and then divided into the blank control group, the PGE2 (prostaglandin E2) group, the anti-miRNA-21 group and the PGE2 + anti-miRNA-21 group and the MTT and the flow cytometry methods were adopted to test the effect of PGE2 or/and anti-miRNA-21 intervention on AGS cell proliferation and apoptosis and the differences to miRNA-21 expression. In addition, the cells were also divided into the blank control group, the PGE2 group, the PGE2 + Perifosine group, the PGE2 + anti-miRNA-21 group and the PGE2 + anti-miRNA-21 + Perifosine group and the MTT and flow cytometry methods were adopted to test the effect of Perifosine intervention on AGS cell proliferation and apoptosis and on PTEN and p-AktmRNA and protein expressions.
Compared with the control group, AGS cell proliferation activity increased significantly, the apoptosis rate decreased and the miRNA-21tmRNA and protein expression increased in the PGE2 group (p < 0.05); compared with the PGE2 group, the AGS cell proliferation rate decreased, the apoptosis rate increased and the miRNA-21mRNA and protein expressions decreased (p < 0.05) in the anti-miRNA-21 group and the PGE2 + anti-miRNA-21 group. In addition, after intervention of Perifosine, the AGS cell proliferation rate decreased, the apoptosis rate increased, the PTEN mRNA and protein expressions increased and the pAktmRNA and protein expressions decreased (p < 0.05).
miRNA-21 may promote the growth of gastric cancer cells by adjusting and controlling PTEN/Akt signal passage mediated PEG2.
研究 miRNA-21 对胃癌 AGS 增殖的刺激作用及其可能的激活机制。
体外培养 AGS 胃癌细胞，分为空白对照组、PGE2（前列腺素 E2）组、anti-miRNA-21 组和 PGE2+anti-miRNA-21 组，采用 MTT 和流式细胞术检测 PGE2 或/和 anti-miRNA-21 干预对 AGS 细胞增殖和凋亡的影响及对 miRNA-21 表达的差异。此外，细胞还分为空白对照组、PGE2 组、PGE2+Perifosine 组、PGE2+anti-miRNA-21 组和 PGE2+anti-miRNA-21+Perifosine 组，采用 MTT 和流式细胞术检测 Perifosine 干预对 AGS 细胞增殖和凋亡及 PTEN 和 p-AktmRNA 和蛋白表达的影响。
与对照组相比，PGE2 组 AGS 细胞增殖活性明显增加，凋亡率降低，miRNA-21tmRNA 和蛋白表达增加（p<0.05）；与 PGE2 组相比，anti-miRNA-21 组和 PGE2+anti-miRNA-21 组 AGS 细胞增殖率降低，凋亡率增加，miRNA-21mRNA 和蛋白表达降低（p<0.05）。此外，经 Perifosine 干预后，AGS 细胞增殖率降低，凋亡率增加，PTENmRNA 和蛋白表达增加，pAktmRNA 和蛋白表达降低（p<0.05）。
miRNA-21 可能通过调节和控制 PEG2 介导的 PTEN/Akt 信号通路促进胃癌细胞的生长。
