Som Suman, Dutta Banik Gourab, Maity Abhijit, Ghosh Chiranjit, Chaudhuri Sujit, Pradhan Manik
a Department of Chemical, Biological and Macro-Molecular Sciences , S. N. Bose National Centre for Basic Sciences , Salt Lake , Kolkata , India.
b Department of Gastroenterology , AMRI Hospital , Salt Lake City , Kolkata , India.
Isotopes Environ Health Stud. 2018 Aug;54(4):435-445. doi: 10.1080/10256016.2018.1467414. Epub 2018 Apr 24.
Helicobacter pylori causes several gastrointestinal diseases and may also contribute to the development of type 2 diabetes (T2D). Several studies suggest that there might be a potential link between H. pylori infection and T2D, but it still remains the subject of debate. Here, we first report the cumulative effect of H. pylori infection and T2D by exploiting the excretion kinetics of C/C and O/O isotope ratios of exhaled breath CO in response to an oral dose of C-enriched glucose in individuals with T2D and non-diabetic controls (NDC) harbouring the H. pylori infection. Using a high-resolution integrated cavity output spectroscopy (ICOS) technique in the infrared region, we observed that the isotopic fractionations of C and O in breath CO are distinctly altered in H. pylori infected T2D patients as well as in H. pylori infected NDC. Several optimal diagnostic cut-off points of C and O isotopes of breath CO were also determined which exhibited the diagnostic sensitivity and specificity of ∼97 % and thus suggesting that breath C and O isotopes might be considered as potential biomarkers for the non-invasive assessment of the gastric pathogen prior to the onset of T2D. This may open a new diagnostic strategy for treating these common diseases in an alternative way.
幽门螺杆菌会引发多种胃肠道疾病，还可能促使2型糖尿病（T2D）的发展。多项研究表明，幽门螺杆菌感染与T2D之间可能存在潜在联系，但这仍是一个有争议的话题。在此，我们通过利用呼气中CO的C/C和O/O同位素比率的排泄动力学，首次报告了幽门螺杆菌感染与T2D的累积效应，该动力学是针对口服富含C的葡萄糖后，T2D患者和携带幽门螺杆菌感染的非糖尿病对照（NDC）人群的呼气情况。我们使用红外区域的高分辨率集成腔输出光谱（ICOS）技术，观察到幽门螺杆菌感染的T2D患者以及幽门螺杆菌感染的NDC人群中，呼气CO中C和O的同位素分馏明显改变。还确定了呼气CO中C和O同位素的几个最佳诊断临界点，其诊断敏感性和特异性约为97%，这表明呼气C和O同位素可被视为在T2D发病前对胃部病原体进行无创评估的潜在生物标志物。这可能会开启一种以替代方式治疗这些常见疾病的新诊断策略。
