人肝脏乙醇脱氢酶β2亚基的互补DNA序列

cDNA sequence of the beta 2-subunit of human liver alcohol dehydrogenase.

作者信息

Ehrig T, von Wartburg J P, Wermuth B

机构信息

Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.

出版信息

FEBS Lett. 1988 Jul 4;234(1):53-5. doi: 10.1016/0014-5793(88)81301-2.

DOI:10.1016/0014-5793(88)81301-2

PMID:2968918

Abstract

A cDNA library of mRNA from a human liver expressing the beta 2-subunit of alcohol dehydrogenase was constructed in lambda gt11. One clone coding for 352 of a total of 374 amino acid residues of the beta 2-subunit was isolated. The sequence differed from that of the beta 1-subunit at one nucleotide position resulting in an Arg/His exchange at position 47 of the peptide chain, in agreement with data from protein sequence analysis [(1984) FEBS Lett. 173, 360-366].

摘要

利用λgt11构建了来自表达乙醇脱氢酶β2亚基的人肝脏mRNA的cDNA文库。分离出一个编码β2亚基总共374个氨基酸残基中352个的克隆。该序列在一个核苷酸位置上与β1亚基的序列不同，导致肽链第47位的精氨酸/组氨酸交换，这与蛋白质序列分析的数据一致[(1984)欧洲生物化学学会联合会快报173, 360 - 366]。