Atkinson M A, Appella E, Corigliano-Murphy M A, Korn E D
Laboratory of Cell Biology, National Heart, Lung and Blood Institute, Bethesda, MD 20892.
FEBS Lett. 1988 Jul 18;234(2):435-8. doi: 10.1016/0014-5793(88)80132-7.
Polyclonal antibodies raised against a synthetic peptide consisting of the last 19 amino acids at the end of the coiled-coil region of the heavy chains inhibited the actin-activated Mg2+-ATPase activity of myosin II and its ability to form filaments. Antibodies against a synthetic peptide corresponding to the 21 adjacent amino acids at the beginning of the non-helical tailpiece, which include the three regulatory phosphorylatable serines, had no effect on either activity.
针对由重链卷曲螺旋区域末端的最后19个氨基酸组成的合成肽产生的多克隆抗体，抑制了肌球蛋白II的肌动蛋白激活的Mg2 + -ATP酶活性及其形成细丝的能力。针对对应于非螺旋尾段起始处21个相邻氨基酸（包括三个可调节磷酸化的丝氨酸）的合成肽的抗体，对这两种活性均无影响。
