来自莫斯科地区的伊丽莎白摇蚊（摇蚊属（摇蚊亚属））新种的形态学描述及DNA条形码分析（双翅目：摇蚊科：直突摇蚊亚科）

Morphological description and DNA barcoding of Chaetocladius (Chaetocladius) elisabethae sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Moscow Region.

作者信息

Makarchenko Eugenyi A, Makarchenko Marina A, Semenchenko Alexander A, Palatov Dmitry M

机构信息

Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia. Far Eastern State Technical Fisheries University, Lugovaya 52b, 690087 Vladivostok, Russia.

出版信息

Zootaxa. 2018 Apr 4;4403(2):378-388. doi: 10.11646/zootaxa.4403.2.9.

DOI:10.11646/zootaxa.4403.2.9

PMID:29690239

Abstract

Illustrated descriptions of the adult male, pupa and fourth instar larva, as well as DNA barcoding results of Chaetocladius (Chaetocladius) elisabethae sp. nov. in comparison with closely related species of Chaetocladius s. str. from the Moscow Region are provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. Comparisons with corresponding regions of COI between C. (s. str.) elisabethae sp. nov. and other species of the subgenus produced K2P genetic distances of 0.11-0.16, values well associated with interspecific variation. The barcodes of the new species were identical to the Chaetocladius sp. 2ES in BOLD systems. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius s. str.

摘要

提供了成年雄性、蛹和四龄幼虫的图示描述，以及新物种伊丽莎白开氏摇蚊（Chaetocladius (Chaetocladius) elisabethae sp. nov.）与莫斯科地区近缘的狭义开氏摇蚊物种的DNA条形码结果。来自线粒体基因细胞色素氧化酶I（COI）片段的658 bp参考条形码序列被用作物种界定的工具。将新物种伊丽莎白开氏摇蚊（狭义）与该亚属其他物种的COI相应区域进行比较，得到的K2P遗传距离为0.11 - 0.16，这些值与种间变异密切相关。新物种的条形码在BOLD系统中与开氏摇蚊2ES物种相同。分子数据还用于重建狭义开氏摇蚊亚属内的系统发育关系。