Mar Win, Kang Peng-Fei, Mao Bin, Wang Yu-Feng
School of Life Sciences, Hubei key laboratory of genetic regulation and integrative biology, Central China Normal University, Wuhan 430079, PR China Department of Zoology, University of Mandalay, Mandalay, Republic of the Union of Myanmar.
Zootaxa. 2018 Feb 28;4388(1):123-132. doi: 10.11646/zootaxa.4388.1.9.
Myanmar is abundant in lakes and rivers, yet only a few investigations on the fauna of shrimps and prawns have been conducted and no molecular characteristics of prawn species have been described. This study reveals the morphologically identification of five freshwater prawn species under the genus Macrobrachium, including M. cavernicola, M. australiense, M. johnsoni, M. josephi and Macrobrachium sp.WMY-2017. As there was no previous record and information concerning with M. australiense, M. johnsoni, M. josephi and Macrobrachium sp. WMY-2017, they were regarded as the first record from Myanmar. A fragment of Mitochondrial Cytochrome Oxidase I Gene (COI) was amplified successfully from three studied species: M. australiense, M. josephi, and Macrobrachium sp.WMY-2017. The interspecific divergences of studied species varied from 0.01 to 0.15. The phylogenetic tree based on COI fragment sequences showed that M. australiense was closely related to M. rosenbergii, while Macrobrachium sp. WMY-2017 was closest to M. josephi. The results of molecular phylogeny has clarified the relationship within the genus Macrobrachium and represents the first step toward understanding the pattern of speciation base on molecular approach in Myanmar.
缅甸湖泊和河流众多，但对虾类动物区系的研究却很少，且尚未描述对虾种类的分子特征。本研究揭示了对罗氏沼虾属五种淡水对虾的形态学鉴定，包括洞穴沼虾、澳大利亚沼虾、约翰逊沼虾、约瑟夫沼虾和WMY - 2017沼虾。由于此前没有关于澳大利亚沼虾、约翰逊沼虾、约瑟夫沼虾和WMY - 2017沼虾的记录和信息，它们被视为缅甸的首次记录。成功从三个研究物种（澳大利亚沼虾、约瑟夫沼虾和WMY - 2017沼虾）中扩增出线粒体细胞色素氧化酶I基因（COI）片段。研究物种的种间差异在0.01至0.15之间。基于COI片段序列的系统发育树表明，澳大利亚沼虾与罗氏沼虾关系密切，而WMY - 2017沼虾与约瑟夫沼虾关系最近。分子系统发育结果阐明了罗氏沼虾属内的关系，代表了在缅甸基于分子方法理解物种形成模式的第一步。
