Department of Horticulture, School of Food Science and Engineering, Foshan University, Foshan 528000, China.
Department of Plant Cell Biology and Bioengineering, Biological Faculty, Belarusian State University, 4 Independence Avenue, 220030 Minsk, Belarus.
Int J Mol Sci. 2018 Apr 23;19(4):1263. doi: 10.3390/ijms19041263.
Ion channels activated by reactive oxygen species (ROS) have been found in the plasma membrane of charophyte , dicotyledon , and , and the monocotyledon . Their activities have been reported in charophyte giant internodes, root trichoblasts and atrichoblasts, pollen tubes, and guard cells. Hydrogen peroxide and hydroxyl radicals are major activating species for these channels. Plant ROS-activated ion channels include inwardly-rectifying, outwardly-rectifying, and voltage-independent groups. The inwardly-rectifying ROS-activated ion channels mediate Ca-influx for growth and development in roots and pollen tubes. The outwardly-rectifying group facilitates K⁺ efflux for the regulation of osmotic pressure in guard cells, induction of programmed cell death, and autophagy in roots. The voltage-independent group mediates both Ca influx and K⁺ efflux. Most studies suggest that ROS-activated channels are non-selective cation channels. Single-channel studies revealed activation of 14.5-pS Ca influx and 16-pS K⁺ efflux unitary conductances in response to ROS. The molecular nature of ROS-activated Ca influx channels remains poorly understood, although annexins and cyclic nucleotide-gated channels have been proposed for this role. The ROS-activated K⁺ channels have recently been identified as products of Stellar K⁺ Outward Rectifier () and Guard cell Outwardly Rectifying K⁺ channel () genes.
已在轮藻、双子叶植物和单子叶植物的质膜中发现了由活性氧 (ROS) 激活的离子通道。它们的活性已在轮藻巨型节间、根毛原基和无毛原基、花粉管和保卫细胞中被报道。过氧化氢和羟基自由基是这些通道的主要激活物质。植物 ROS 激活的离子通道包括内向整流、外向整流和电压无关的组。内向整流的 ROS 激活的离子通道介导 Ca 流入，以促进根和花粉管的生长和发育。外向整流组促进 K⁺外排，以调节保卫细胞中的渗透压、诱导程序性细胞死亡和根中的自噬。电压无关的组介导 Ca 流入和 K⁺外排。大多数研究表明，ROS 激活的通道是非选择性阳离子通道。单通道研究表明，ROS 激活可响应 ROS 激活 14.5-pS Ca 流入和 16-pS K⁺外排单通道电导。尽管已经提出 annexin 和环核苷酸门控通道具有这种作用，但 ROS 激活的 Ca 流入通道的分子性质仍知之甚少。ROS 激活的 K⁺通道最近被鉴定为星状 K⁺外向整流器 () 和保卫细胞外向整流 K⁺通道 () 基因的产物。
