Laboratory of Enzymatic Biotechnology, Department of Pharmacy, State University of Maringá (UEM), Maringá, PR, Brazil.
Laboratory of Enzymatic Biotechnology, Department of Pharmacy, State University of Maringá (UEM), Maringá, PR, Brazil.
Carbohydr Polym. 2018 Jul 15;192:19-27. doi: 10.1016/j.carbpol.2018.03.035. Epub 2018 Mar 15.
The study comparatively evaluated diverse strategic models of cyclodextrin (CD) production by the CGTase of Bacillus firmus strain 37: continuous production and repetitive batches in ultrafiltration systems; immobilization of CGTase on curdlan and vegetable sponge natural supports; the use of the glycyrrhizin complexing agent to modulate CGTase selectivity in favor of γ-CD production. All strategies had in common the possibility of separation of CGTase from its inhibitory products and its reuse. In the continuous production model, at 48 h of assay, the highest productivity and selectivity for β-CD were obtained, 1.47 mmol/L/h and 92.8%, respectively. Glycyrrhizin was able to modulate the production of γ-CD with selectivity of 61.2% for 30-h batches. The comparative evaluation of the different strategic models for obtaining CDs showed particularities that should be considered, and most of the models studied returned satisfactory yields as well as excellent selectivity.
该研究通过纤维芽胞杆菌 37 株的环糊精糖基转移酶（CGTase）对不同的环糊精（CD）生产策略模型进行了比较评估：超滤系统中的连续生产和重复批次；CGTase 在几丁聚糖和蔬菜海绵天然载体上的固定化；使用甘草酸复合物调节剂来调节 CGTase 的选择性，有利于 γ-CD 的生产。所有策略的共同点是能够分离 CGTase 及其抑制产物并重复使用。在连续生产模型中，在 48 小时的测定中，β-CD 的最高生产力和选择性分别为 1.47mmol/L/h 和 92.8%。甘草酸能够调节 γ-CD 的生产，其选择性为 61.2%，批次时间为 30 小时。获得 CD 的不同策略模型的比较评估显示出需要考虑的特殊性，并且所研究的大多数模型都返回了令人满意的产量和极好的选择性。
