Chandra T Jaya
Department of Microbiology, GSL Medical College, Rajahmundry, Andhra Pradesh, India.
J Lab Physicians. 2018 Apr-Jun;10(2):135-139. doi: 10.4103/JLP.JLP_145_17.
To evaluate the efficacy of one-sputum sample two-smear approach for the diagnosis of pulmonary tuberculosis (PT).
Data from January 2012 to December 2015 were analyzed to find (1) number of smear positives (SPs) by spot (S) sample with one and two smears; (2) number of SPs by morning (M) sample with one and two smears; and (iii) number of SPs by two samples with two smears, that is, same-day (SS) and spot morning (SM) approaches. The Chi-square test was used to evaluate the statistical difference in SP cases.
With one-sample two-smear approach, the smear positivity (SPT) was 87% and 87.5%, for S and M samples, respectively, for Ziehl-Neelsen (ZN) staining; whereas, SPT was 96% and 97%, respectively, for S and M samples, for fluorescent staining (FS) technique. With two-sample two-smear approach, for ZN staining, SPT was 89% each and for FS technique, SPT was 97% and 99%, respectively, for SS and SM approaches. The difference was not statistically significant ( > 0.05) between one- and two-sample approaches in the staining techniques.
Significant number of SP cases are identified by S sample two-smear approach. Thus, the World Health Organization/Revised National Tuberculosis Control Programme can initiate S sample two-smear approach for the diagnosis of PT.
评估单份痰标本两次涂片法诊断肺结核（PT）的疗效。
分析2012年1月至2015年12月的数据，以确定：（1）即时（S）痰标本一次和两次涂片的涂片阳性（SP）数；（2）晨痰（M）标本一次和两次涂片的SP数；以及（3）两份标本两次涂片的SP数，即同日（SS）和即时晨痰（SM）法。采用卡方检验评估SP病例的统计学差异。
对于萋-尼（ZN）染色，采用单份标本两次涂片法时，S和M标本的涂片阳性率（SPT）分别为87%和87.5%；而对于荧光染色（FS）技术，S和M标本的SPT分别为96%和97%。对于两份标本两次涂片法，ZN染色时，SS和SM法的SPT均为89%，FS技术时，SS和SM法的SPT分别为97%和99%。两种涂片技术的单份和两份标本方法之间差异无统计学意义（>0.05）。
通过S标本两次涂片法可发现大量SP病例。因此，世界卫生组织/修订后的国家结核病控制规划可采用S标本两次涂片法诊断PT。
