曼氏血吸虫50千道尔顿潜在表面抗原编码基因的克隆

Cloning of the gene encoding a 50 kilodalton potential surface antigen of Schistosoma mansoni.

作者信息

Havercroft J C, Huggins M C, Nene V, Dunne D W, Richardson B A, Taylor D W, Butterworth A E

机构信息

Department of Pathology, University of Cambridge, U.K.

出版信息

Mol Biochem Parasitol. 1988 Jul;30(1):83-8. doi: 10.1016/0166-6851(88)90135-1.

DOI:10.1016/0166-6851(88)90135-1

PMID:2969455

Abstract

A cDNA library was constructed from the mRNA of adult worms of Schistosoma mansoni, in the expression vector lambda gt11. This library was screened with a pool of sera raised against either soluble egg antigens or purified schistosomulum tegumental membranes. An antiserum raised against the fusion protein of one clone immunoprecipitated a 45 kDa polypeptide from the in vitro translation products of adult worm mRNA and recognised a 50 kDa antigen in homogenates of adult worms. This serum gave positive fluorescence of the surface of schistosomula in indirect immunofluorescence assays and was able to mediate killing of schistosomula by human eosinophils in vitro, suggesting that this clone contained part of a gene encoding a surface antigen.

摘要

从曼氏血吸虫成虫的mRNA构建了一个cDNA文库，该文库构建于表达载体λgt11中。用针对可溶性虫卵抗原或纯化的童虫体表膜制备的一组血清对该文库进行筛选。一种针对一个克隆的融合蛋白产生的抗血清，从成虫mRNA的体外翻译产物中免疫沉淀出一条45 kDa的多肽，并在成虫匀浆中识别出一种50 kDa的抗原。在间接免疫荧光试验中，该血清使童虫表面产生阳性荧光，并且能够在体外介导人嗜酸性粒细胞对童虫的杀伤作用，这表明该克隆包含编码一种表面抗原的基因的一部分。