Departments of Neuroscience & Physiology, Laboratory of Stress Hormones & Plasticity, Institut de Génomique Fonctionnelle, INSERM, CNRS, University of Montpellier, 34093 Montpellier, France.
Department of Neuroscience, Laboratory of Cerebrovascular Mechanisms of Brain Disorders, Institut de Génomique Fonctionnelle, INSERM, CNRS, University of Montpellier, 34093 Montpellier, France.
Cell Rep. 2018 Apr 24;23(4):1045-1059. doi: 10.1016/j.celrep.2018.03.110.
Reorganization of the neurovascular unit has been suggested in the epileptic brain, although the dynamics and functional significance remain unclear. Here, we tracked the in vivo dynamics of perivascular mural cells as a function of electroencephalogram (EEG) activity following status epilepticus. We segmented the cortical vascular bed to provide a size- and type-specific analysis of mural cell plasticity topologically. We find that mural cells are added and removed from veins, arterioles, and capillaries after seizure induction. Loss of mural cells is proportional to seizure severity and vascular pathology (e.g., rigidity, perfusion, and permeability). Treatment with platelet-derived growth factor subunits BB (PDGF-BB) reduced mural cell loss, vascular pathology, and epileptiform EEG activity. We propose that perivascular mural cells play a pivotal role in seizures and are potential targets for reducing pathophysiology.
已有研究提示癫痫脑中存在神经血管单元重构，但其动态变化及功能意义仍不清楚。本研究通过在癫痫发作后记录脑电活动，跟踪血管周细胞的活体动力学变化。我们对皮质血管床进行分割，提供了一种基于大小和类型的血管周细胞拓扑可塑性分析方法。我们发现，在癫痫诱导后，血管周细胞可从静脉、小动脉和毛细血管中增加或消失。血管周细胞丢失与癫痫发作严重程度和血管病理（如僵硬、灌注和通透性）呈比例关系。血小板衍生生长因子亚基 BB（PDGF-BB）治疗可减少血管周细胞丢失、血管病理和癫痫样脑电活动。我们提出，血管周细胞在癫痫发作中起关键作用，是减少病理生理学改变的潜在靶点。
