Matharu Rupy Kaur, Porwal Harshit, Ciric Lena, Edirisinghe Mohan
Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Department of Civil, Environmental & Geomatic Engineering, University College London, Chadwick Building, Gower Street, London WC1E 6BT, UK.
Interface Focus. 2018 Jun 6;8(3):20170058. doi: 10.1098/rsfs.2017.0058. Epub 2018 Apr 20.
A novel class of ultra-thin fibres, which affect microbial growth, were explored. The microbial properties of poly(methyl methacrylate) fibres containing 2, 4 and 8 wt% of graphene nanoplatelets (GNPs) were studied. GNPs were dispersed in a polymeric solution and processed using pressurized gyration. Electron microscopy was used to characterize GNP and fibre morphology. Scanning electron microscopy revealed the formation of beaded porous fibres. GNP concentration was found to dictate fibre morphology. As the GNP concentration increased, the average fibre diameter increased from 0.75 to 2.71 µm, while fibre porosity decreased. Gram-negative bacteria and were used to investigate the properties of 2, 4 and 8 wt% GNP-loaded fibres. GNP-loaded fibres (0 wt%) were used as the negative control. The fibres were incubated for 24 h with the bacteria; bacterial colony-forming units were enumerated by adopting the colony-counting method. The presence of 2 and 4 wt% GNP-loaded fibres promoted microbial growth, while 8 wt% GNP-loaded fibres showed antimicrobial activity. These results indicate that the minimum inhibitory concentration of GNPs required within a fibre is 8 wt%.
研究了一类影响微生物生长的新型超薄纤维。研究了含有2%、4%和8%（重量）石墨烯纳米片（GNP）的聚甲基丙烯酸甲酯纤维的微生物特性。将GNP分散在聚合物溶液中，并采用加压旋转法进行处理。利用电子显微镜对GNP和纤维形态进行表征。扫描电子显微镜显示形成了珠状多孔纤维。发现GNP浓度决定纤维形态。随着GNP浓度增加，平均纤维直径从0.75微米增加到2.71微米，而纤维孔隙率降低。使用革兰氏阴性菌来研究负载2%、4%和8%（重量）GNP的纤维的性能。未负载GNP的纤维（0%重量）用作阴性对照。将纤维与细菌孵育24小时；采用菌落计数法计算细菌菌落形成单位。负载2%和4%（重量）GNP的纤维的存在促进了微生物生长，而负载8%（重量）GNP的纤维表现出抗菌活性。这些结果表明，纤维中所需GNP的最低抑菌浓度为8%（重量）。
