[电针“长强”（GV 1）对急性脊髓损伤大鼠神经生长因子和脑源性神经营养因子表达的影响]
[Effects of electroacupuncture at "Changqiang" (GV 1) on expression of nerve growth factor and brain derived neurotrophic factor in rats after acute spinal cord injury].
作者信息
Huang Zhiqiang, Liu Yu, Su Zhaoyuan, Su Jiafu, Wu Qiang
机构信息
Fujian University of TCM, Fuzhou 350122, China; Quanzhou TCM Hospital Affiliated to Fujian University of TCM, Quanzhou 362000.
Fujian University of TCM, Fuzhou 350122, China.
出版信息
Zhongguo Zhen Jiu. 2018 Apr 12;38(4):399-404. doi: 10.13703/j.0255-2930.2018.04.015.
OBJECTIVE
To observe the effects of electroacupuncture (EA) at"Changqiang"(GV 1) on expression of nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF) in rats after acute spinal cord injury (ASCI), and to explore the mechanism of EA at"Changqiang"(GV 1) on ASCI.
METHODS
Twenty-four adult female SD rats were randomly divided into an EA group, a model group and a sham operation group, 8 rats in each one. The rats in the sham operation group were treated with laminectomy to expose the spinal cord without any strike. The rats in the model group and EA group were treated with modified Allen's method to establish ASCI model. After model was established, the rats in the EA group were treated with EA at"Changqiang"(GV 1), once a day for continuous 7 days. The rats in the sham operation group and model group were treated with immobilization, once a day, without any other interventions. The basso beattie bresnahan (BBB) was evaluated 1, 3, 5, 7 days after operation. 7 days after operation, the rats were sacrificed with perfusion and the spinal cord was embedded with paraffin. The morphological changes of spinal cord and neuron were observed by Nissl's staining method; the expressions of NGF and BDNF were detected by immune fluorescence method.
RESULTS
3 days, 5 days and 7 days after operation, the BBB scores in the EA group were higher than those in the model group (<0.05, <0.01). The Nissl's staining indicated the gray matter of spinal cord was butterfly-shaped with complete structure and clear boundaries between the gray and white matter; the tabby-shaped Nissl bodies were observed in cytoplasm. There were incomplete gray nucleus, big and saturate local stasis plaque. Compared with the model group, the smallerarea of blood stasis, less severity of neuron edema, better morphology of neuron and no vacuole change were observed in the EA group. The immune fluorescence results indicated the expressions of NGF and BDNF in the model group and EA group were higher than those in the sham operation group (all <0.01); the expressions of NGF and BDNF in the EA group were higher than those in the model group (both <0.01).
CONCLUSION
EA at"Changqiang"(GV 1) could improve the expression of NGF and BDNF and increase the score of BBB in rats with ASCI, which is beneficial to the repair of ASCI.
目的
观察针刺“长强”（GV 1）穴对急性脊髓损伤（ASCI）大鼠神经生长因子（NGF）和脑源性神经营养因子（BDNF）表达的影响，探讨针刺“长强”（GV 1）穴治疗ASCI的机制。
方法
将24只成年雌性SD大鼠随机分为针刺组、模型组和假手术组，每组8只。假手术组行椎板切除术暴露脊髓但不进行打击；模型组和针刺组采用改良Allen's法制备ASCI模型。造模成功后，针刺组大鼠针刺“长强”（GV 1）穴，每日1次，连续7天；假手术组和模型组大鼠固定，每日1次，不进行其他干预。术后1、3、5、7天进行Basso Beattie Bresnahan（BBB）评分。术后7天，灌注处死大鼠，脊髓石蜡包埋。采用尼氏染色法观察脊髓形态及神经元变化；采用免疫荧光法检测NGF和BDNF的表达。
结果
术后3天、5天、7天，针刺组BBB评分高于模型组（<0.05，<0.01）。尼氏染色显示脊髓灰质呈蝶形，结构完整，灰质与白质界限清晰；胞质内可见板层状尼氏体。存在灰质核不完整、局部淤血斑大且密集。与模型组相比，针刺组淤血面积较小，神经元水肿程度较轻，神经元形态较好，无空泡改变。免疫荧光结果显示，模型组和针刺组NGF和BDNF的表达高于假手术组（均<0.01）；针刺组NGF和BDNF的表达高于模型组（均<0.01）。
结论
针刺“长强”（GV 1）穴可提高ASCI大鼠NGF和BDNF的表达，增加BBB评分，有利于ASCI的修复。
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