Tomavo S, Ouaissi M A, Dubremetz J F, Capron A, Vernes A
Unité 42 INSERM, Villeneuve-d'Ascq.
Ann Parasitol Hum Comp. 1988;63(1):22-7. doi: 10.1051/parasite/198863122.
A fibronectin binding protein (FnBp) was identified in 3H isoleucine labeled P. falciparum schizonts using affinity chromatography on human fibronectin (Fn) coupled to Sepharose 4B. After incubation of Nonidet-P 40 parasite lysate with Fn-Sepharose, elution was performed with SDS-PAGE buffer. Analysis of FnBp by SDS-PAGE demonstrated a major band which migrated with an apparent Mr of 70,000 under reducing conditions. This band was not found when human or rabbit IgG coupled Sepharose 4B were used instead of Fn as control.
利用结合了琼脂糖凝胶4B的人纤连蛋白（Fn）进行亲和层析，在3H异亮氨酸标记的恶性疟原虫裂殖体中鉴定出一种纤连蛋白结合蛋白（FnBp）。将Nonidet-P 40寄生虫裂解物与纤连蛋白琼脂糖凝胶孵育后，用SDS-PAGE缓冲液进行洗脱。通过SDS-PAGE对FnBp进行分析，结果显示在还原条件下有一条主要条带，其表观分子量为70,000。当使用结合了人或兔IgG的琼脂糖凝胶4B作为对照代替Fn时，未发现这条带。
