Kroese Michiel V, Beckers Lisa, Bisselink Yvette J W M, Brasseur Sophie, van Tulden Peter W, Koene Miriam G J, Roest Hendrik I J, Ruuls Robin C, Backer Jantien A, IJzer Jooske, van der Giessen Joke W B, Willemsen Peter T J
1 Wageningen Bioveterinary Research of Wageningen University and Research (WBVR), Edelhertweg 15, 8200 AB Lelystad, the Netherlands.
2 National Institute of Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Antonie van Leeuwenhoeklaan 9, 3520 BA Bilthoven, the Netherlands.
J Wildl Dis. 2018 Jul;54(3):439-449. doi: 10.7589/2017-05-097. Epub 2018 Apr 26.
Brucellosis is a zoonotic disease with terrestrial or marine wildlife animals as potential reservoirs for the disease in livestock and human populations. The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. The overall prevalence of Brucella spp. antibodies in sera from healthy wild grey seals ( Halichoerus grypus; n=11) and harbor seals ( Phoca vitulina; n=40), collected between 2007 and 2013 ranged from 25% to 43%. Additionally, tissue samples of harbor seals collected along the Dutch shores between 2009 and 2012, were tested for the presence of Brucella spp. In total, 77% (30/39) seals were found to be positive for Brucella by IS 711 real-time PCR in one or more tissue samples, including pulmonary nematodes. Viable Brucella was cultured from 40% (12/30) real-time PCR-positive seals, and was isolated from liver, lung, pulmonary lymph node, pulmonary nematode, or spleen, but not from any PCR-negative seals. Tissue samples from lung and pulmonary lymph nodes were the main source of viable Brucella bacteria. All isolates were typed as B. pinnipedialis by multiple-locus variable number of tandem repeats analysis-16 clustering and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and of sequence type ST25 by multilocus sequence typing analysis. No correlation was observed between Brucella infection and pathology. This report displays the isolation and identification of B. pinnipedialis in marine mammals in the Dutch part of the Atlantic Ocean.
布鲁氏菌病是一种人畜共患病，陆地或海洋野生动物可能是家畜和人类群体中该疾病的潜在宿主。本研究的主要目的是评估荷兰海岸沿线海洋哺乳动物中是否存在海狗布鲁氏菌，并观察海狗布鲁氏菌的存在与受感染动物中伴随病理之间的可能相关性。2007年至2013年期间采集的健康野生灰海豹（Halichoerus grypus；n = 11）和港海豹（Phoca vitulina；n = 40）血清中布鲁氏菌属抗体的总体患病率在25%至43%之间。此外，对2009年至2012年期间在荷兰海岸采集的港海豹组织样本进行了布鲁氏菌属检测。通过IS 711实时PCR检测，总共77%（30/39）的海豹在一个或多个组织样本（包括肺线虫）中布鲁氏菌呈阳性。从40%（12/30）实时PCR阳性的海豹中培养出了活的布鲁氏菌，这些菌从肝脏、肺、肺淋巴结、肺线虫或脾脏中分离得到，但未从任何PCR阴性的海豹中分离得到。肺和肺淋巴结的组织样本是活布鲁氏菌的主要来源。通过多位点可变串联重复序列分析-16聚类和基质辅助激光解吸电离飞行时间质谱法将所有分离株鉴定为海狗布鲁氏菌，通过多位点序列分型分析鉴定为序列型ST25。未观察到布鲁氏菌感染与病理之间的相关性。本报告展示了在大西洋荷兰部分的海洋哺乳动物中海狗布鲁氏菌的分离和鉴定。
