Ammendola R, Russo T, Cimino F
Istituto di Scienze Biochimiche, II Facoltà di Medicina e Chirurgia, Napoli.
Ital J Biochem. 1988 Mar-Apr;37(2):85-90.
The identification and characterization of a mouse tRNA(Trp) pseudogene is reported. A synthetic oligonucleotide (31 mer), identical with the 3' half of a tRNA(Trp), was used to examine three mouse lambda clones that are known to contain clusters of tRNA genes. A fragment of one of these lambda clones strongly hybridizes to the oligonucleotide; the sequence of this region shows the presence of a gene significantly homologous to the chick tRNA(Trp). However two point mutations and a three base deletion prevent the folding of a possible transcript of this gene. The presence of a conserved promoter sequence for RNA polymerase III, that should allow the transcription of this gene, does not ensure the transcription of the gene, at least in our in vitro system.
报道了一个小鼠tRNA(Trp)假基因的鉴定和特征分析。一个与tRNA(Trp) 3' 端一半相同的合成寡核苷酸（31聚体）被用于检测三个已知包含tRNA基因簇的小鼠λ克隆。其中一个λ克隆的片段与该寡核苷酸强烈杂交；该区域的序列显示存在一个与鸡tRNA(Trp)显著同源的基因。然而，两个点突变和一个三碱基缺失阻止了该基因可能转录本的折叠。RNA聚合酶III保守启动子序列的存在，本应允许该基因转录，但至少在我们的体外系统中，这并不能确保该基因的转录。
