通过与合成寡核苷酸杂交分离人甘油醛-3-磷酸脱氢酶基因。
Isolation of the human glyceraldehyde-3-phosphate dehydrogenase gene by hybridization with synthetic oligonucleotides.
作者信息
Martinelli R, Arcari P, Salvatore F
机构信息
Istituto di Scienze Biochimiche, II Facoltà di Medicina e Chirurgia, Napoli.
出版信息
Ital J Biochem. 1988 Mar-Apr;37(2):91-5.
PMID:2969876
Abstract
By screening a human genomic library from human lymphocyte DNA cloned in EMBL 3 vector with synthetic oligonucleotides homologous to the human GAPD cDNA 3'-non-coding region as probes, a unique lambda recombinant clone (EMBL-G5) was isolated at the Tm value. Preliminary restriction analysis and sequence data proved that this recombinant clone is the human structural gene for the glyceraldehyde- 3-phosphate dehydrogenase.
摘要
通过以与人甘油醛-3-磷酸脱氢酶（GAPD）cDNA 3'非编码区同源的合成寡核苷酸为探针，筛选克隆于EMBL 3载体的人淋巴细胞DNA构建的人类基因组文库，在解链温度（Tm值）下分离出一个独特的λ重组克隆（EMBL-G5）。初步的限制性分析和序列数据证明，该重组克隆是甘油醛-3-磷酸脱氢酶的人类结构基因。
Zotero 插件