Institute of Precision Machinery, Zhejiang Normal University, Jinhua 321004, Zhejiang Province, China; Graduate School of Mechanical Engineering, Division of Artificial System Science, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522 Japan.
Department of Information and Computer Engineering, National Institute of Technology, Kisarazu College, 2-11-1 Kiyomidai-Higashi, Kisarazu, Chiba 292-0041 Japan.
Biosens Bioelectron. 2018 Jul 30;112:79-85. doi: 10.1016/j.bios.2018.04.020. Epub 2018 Apr 12.
Red blood cells (RBCs) aggregability A of coagulating blood in extracorporeal circulation system has been investigated under the condition of pulsatile flow. Relaxation frequency f from the multiple-frequency electrical impedance spectroscopy is utilized to obtain RBCs aggregability A. Compared with other methods, the proposed multiple-frequency electrical impedance method is much easier to obtain non-invasive measurement with high speed and good penetrability performance in biology tissues. Experimental results show that, RBCs aggregability A in coagulating blood falls down with the thrombus formation while that in non-coagulation blood almost keeps the same value, which has a great agreement with the activated clotting time (ACT) fibrinogen concertation (F) tests. Modified Hanai formula is proposed to quantitatively analyze the influence of RBCs aggregation on multiple-frequency electrical impedance measurement. The reduction of RBCs aggregability A is associated with blood coagulation reaction, which indicates the feasibility of the high speed, compact and cheap on-line thrombus measurement biosensors in extracorporeal circulation systems.
已在脉动流条件下研究了体外循环系统中凝血血液的红细胞（RBC）聚集性 A。利用多频电阻抗光谱法的弛豫频率 f 获得 RBC 聚集性 A。与其他方法相比，所提出的多频电阻抗方法更容易获得非侵入性测量，具有高速和良好的生物组织穿透性能。实验结果表明，凝血中 RBC 聚集性 A 随血栓形成而下降，而未凝固血液中的 RBC 聚集性 A 几乎保持相同值，这与激活凝血时间（ACT）纤维蛋白原浓度（F）测试结果非常吻合。提出了修正的 Hanai 公式来定量分析 RBC 聚集对多频电阻抗测量的影响。RBC 聚集性 A 的降低与血液凝固反应有关，这表明在体外循环系统中高速、紧凑且廉价的在线血栓测量生物传感器具有可行性。
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