血液在同基因子宫内膜异位症小鼠模型中早期子宫内膜-腹膜相互作用中的作用。
The role of blood in early endometrial-peritoneal interactions in a syngeneic mouse model of endometriosis.
作者信息
Kobayashi Ayako, Maegawa Masahiko, Yamamoto Satoshi, Ugumori Natsuyo, Kasai Yuka, Tani Anna, Uemura Hirokazu, Kuwahara Akira, Matsuzaki Toshiya, Yasui Toshiyuki, Furumoto Hiroyuki, Kamada Masaharu, Irahara Minoru
机构信息
Department of Obstetrics and Gynecology, Institute of Health Biosciences The University of Tokushima Graduate School 770-8503 Tokushima Japan.
Department of Obstetrics and Gynecology Tokushima Prefectural Central Hospital 1-10-3 Kuramoto-cho 770-8539 Tokushima Japan.
出版信息
Reprod Med Biol. 2010 Sep 2;10(1):15-20. doi: 10.1007/s12522-010-0065-2. eCollection 2011 Mar.
PURPOSE
The aim of this study was to clarify the role of blood in the early stage of development of endometriotic lesions by developing a syngeneic transplantation model using immunocompetent mice.
METHODS
Endometriotic lesions were induced in C57BL/6 mice by an intraperitoneal injection of endometrial fragments plus saline or endometrial fragments plus blood. Some endometrial fragments plus blood were injected with heparin, hirudin or tissue plasminogen activator (tPA). Endometriotic lesions on days 1, 3 and 5 were evaluated by gross and microscopic findings.
RESULTS
The areas of endometriotic lesions in the blood group (6.4 ± 1.7 mm) were significantly larger than those in the saline group (0.5 ± 0.3 mm). The areas of endometriotic lesions were significantly reduced by the addition of heparin, hirudin or tPA. On day 1, endometriotic lesions in the blood group were observed on the peritoneum in five of the six mice. Endometriotic lesions on days 3 and 5 were significantly larger than those on day 1. On day 5, endometriotic lesions appeared cystic in all the mice.
CONCLUSIONS
Blood accelerates the early stage of development of endometriotic lesions when endometrial fragments plus blood are injected. Blood property might be involved in early endometrial-peritoneal interactions.
目的
本研究的目的是通过使用具有免疫活性的小鼠建立同基因移植模型，阐明血液在子宫内膜异位症病变早期发展中的作用。
方法
通过腹腔注射子宫内膜碎片加生理盐水或子宫内膜碎片加血液，在C57BL/6小鼠中诱导子宫内膜异位症病变。一些子宫内膜碎片加血液注射了肝素、水蛭素或组织纤溶酶原激活剂（tPA）。通过大体和显微镜检查结果评估第1、3和5天的子宫内膜异位症病变。
结果
血液组子宫内膜异位症病变面积（6.4±1.7mm）明显大于生理盐水组（0.5±0.3mm）。添加肝素、水蛭素或tPA可使子宫内膜异位症病变面积显著减小。在第1天，六只小鼠中有五只在腹膜上观察到血液组的子宫内膜异位症病变。第3天和第5天的子宫内膜异位症病变明显大于第1天。在第5天，所有小鼠的子宫内膜异位症病变均呈囊性。
结论
当注射子宫内膜碎片加血液时，血液会加速子宫内膜异位症病变的早期发展。血液特性可能参与早期子宫内膜与腹膜的相互作用。
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