Hashimoto Shu, Suzuki Nao, Ishizuka Bunpei, Morimoto Yoshiharu
IVF Namba Clinic 1-17-28 Minami-horie, Nishi-ku 5500015 Osaka Japan.
Department of Obstetrics and Gynecology St Marianna University School of Medicine 2-16-1 Sugao, Miyamae-ku 2168511 Kawasaki Kanagawa Japan.
Reprod Med Biol. 2011 May 15;10(3):161-169. doi: 10.1007/s12522-011-0088-3. eCollection 2011 Sep.
To preserve the fertility of patients who undergo chemotherapy and/or radiotherapy, procedures for cryopreservation of female germ cells have been investigated. Cyropreservation methods differ according to follicle stage because the mammalian ovary contains a large number of oocytes at different growth stages. Follicles at very early stages, for example the primordial and primary stages, are usually cryopreserved within ovarian cortical tissue because they need surrounding somatic cells for subsequent development. In contrast, fully-grown oocytes in Graafian follicles are cryopreserved without any other cells at the metaphase II stage. Recently, ultra-rapid cooling was incorporated into cryopreservation procedures for human ovaries. In this review, we describe oocyte freezing, the development of ultra-rapid cooling systems for ovarian tissues, freezing of human ovaries, and ovarian transplantation.
为了保留接受化疗和/或放疗患者的生育能力，人们对女性生殖细胞的冷冻保存方法进行了研究。由于哺乳动物卵巢中含有大量处于不同生长阶段的卵母细胞，冷冻保存方法会因卵泡阶段而异。例如，处于原始和初级阶段等非常早期的卵泡，通常在卵巢皮质组织内进行冷冻保存，因为它们后续发育需要周围的体细胞。相比之下，格拉夫卵泡中完全成熟的卵母细胞在减数第二次分裂中期无需其他细胞即可进行冷冻保存。最近，超快速冷却已被纳入人类卵巢的冷冻保存程序。在本综述中，我们描述了卵母细胞冷冻、卵巢组织超快速冷却系统的发展、人类卵巢冷冻以及卵巢移植。
