Hirabayashi Masumi, Hochi Shinichi
Center for Genetic Analysis of Behavior National Institute for Physiological Sciences Higashiyama 4-1, Myodaiji-cho 444-8787 Okazaki Aichi Japan.
Division of Applied Biology, Faculty of Textile Science and Technology Shinshu University Ueda Nagano Japan.
Reprod Med Biol. 2011 Jun 24;10(4):231-238. doi: 10.1007/s12522-011-0095-4. eCollection 2011 Dec.
Over the past 25 years, the reverse genetic approach including precise and conditional replacement or loss of gene function at a specific locus was considered possible only in mice due to the absence of embryonic stem (ES) or induced pluripotent stem (iPS) cell lines in other species. Recently, however, stem cell technology in rats has become available for biomedical research. In this paper we overview the recent progress of rat ES and iPS cell technology. Starting from the establishment of rat ES cells, the use of ES cells for foreign gene transfer and endogenous gene knock-out is discussed, followed by the successful establishment of rat iPS cells and the generation of an iPS cell-derived organ via interspecific blastocyst complementation. Finally, the possible contribution of rat stem cell technology to reproductive medicine is described.
在过去25年里，由于其他物种缺乏胚胎干细胞（ES）或诱导多能干细胞（iPS）系，包括在特定基因座精确且有条件地替换或缺失基因功能的反向遗传学方法仅在小鼠中可行。然而，最近大鼠的干细胞技术已可用于生物医学研究。在本文中，我们概述了大鼠ES和iPS细胞技术的最新进展。从大鼠ES细胞的建立开始，讨论了ES细胞在外源基因转移和内源基因敲除中的应用，随后是大鼠iPS细胞的成功建立以及通过种间囊胚互补产生iPS细胞来源的器官。最后，描述了大鼠干细胞技术对生殖医学可能的贡献。
