Saito Natsuho, Yamashita Yoshiki, Ono Yoshihiro, Higuchi Yoko, Hayashi Atsushi, Yoshida Yoko, Yamamoto Hikaru, Kawabe Sachiko, Kamada Mika, Terai Yoshito, Ohmichi Masahide
Department of Obstetrics and Gynecology Osaka Medical College 2-7 Daigakumachi 569-8686 Takatsuki Osaka Japan.
Reprod Med Biol. 2013 Apr 17;12(3):99-104. doi: 10.1007/s12522-013-0147-z. eCollection 2013 Jul.
Examination of the mitochondrial mRNA expression in granulosa cells from an unspecified population of infertile patients to evaluate whether recombinant follicle stimulating hormone (recFSH) is more effective in producing higher quality embryo rates compared with human menopausal gonadotropin (hMG).
Thirty-nine patients who underwent the in vitro fertilization and embryo transfer program were retrospectively examined. Patients were administered recFSH ( = 18) or hMG ( = 20) in a long protocol where GnRH agonist was used. Granulosa cells were obtained during oocyte retrieval and examined for mitochondria mRNA expression ratio against GAPDH. Expressions of mitochondria mRNA were evaluated by real-time PCR analysis.
The high-quality embryo rate in the hMG cycle was higher than in the recFSH cycle, and the total dose of hMG showed a positive correlation with the expression level of mitochondrial genes in granulosa cells. Moreover, mitochondria mRNA expression was higher in the hMG cycle than in the recFSH cycle.
Compared with recFSH, hMG induces a higher mitochondrial gene expression ratio in granulosa cells at the time of oocyte retrieval and, therefore, may lead to higher quality embryo rates.
检测未明确的不孕患者群体颗粒细胞中线粒体mRNA的表达，以评估重组促卵泡激素（recFSH）与绝经期促性腺激素（hMG）相比，在产生更高质量胚胎率方面是否更有效。
回顾性检查39例行体外受精和胚胎移植程序的患者。患者在使用GnRH激动剂的长方案中接受recFSH（n = 18）或hMG（n = 20）治疗。在取卵时获取颗粒细胞，并检测其线粒体mRNA与GAPDH的表达比率。通过实时PCR分析评估线粒体mRNA的表达。
hMG周期的高质量胚胎率高于recFSH周期，且hMG的总剂量与颗粒细胞中线粒体基因的表达水平呈正相关。此外，hMG周期的线粒体mRNA表达高于recFSH周期。
与recFSH相比，hMG在取卵时可诱导颗粒细胞中线粒体基因表达比率更高，因此可能导致更高质量的胚胎率。
